Journal of Gastrointestinal & Digestive System
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
The side population (SP) is thought enriched with hepatocellular cancer stem cells (HCSCs); however, it?s properties has
not been fully explored in fresh tissues of hepatocellular cancer (HCC).
Hoechst33342 was used to isolate the SP cells from HCC cell line HepG2 and fresh tumor tissues of 21 HCC patients by
flow cytometry. Properties of the SP cells were established by cell proliferation assay, capacity to form spheroids in the serum-free
medium, invasion and migration assays, tumorigenicity in NOD/SCID mice and mRNA microarray analysis.
SP cells were observed in HepG2 and 18 of the 21 fresh tissue samples. SP cells showed higher abilities of forming
spheroids, invasion and migration, and there was no significant difference in cells proliferation between the two groups. Tumors
could generate from SP but not non-SP (NSP) cells in a low dose of subcutaneous injection to the NOD/SCID mice (5?102cells/
mouse). The mRNA microarray analysis in HepG2 SP cells revealed that 2057 gene expressions were up-regulated and 3189
Our work indicates that SP cells can be sorted from HepG2 and HCC fresh samples. Compared with NSP cells, SP
has the properties of higher invasion, migration and stemness.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals