This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.
Gastric cancer (GC) is the second most lethal malignancy worldwide and the leading cause of death relating
to neoplastic disease in Chile. Authors have reported that PI3K/AKT/mTOR pathway could play an important role in the
development of GC, moreover, some microRNAs (miRs) could regulate post-transcriptionally the gene expression of this
pathway. The aim of this study was to evaluate both the protein expression of the components of PI3K/AKT/mTOR pathway by
immunohistochemistry (IHC) and the expression of miR-125b and miR-451 in a cohort of GC cases.
The protein expression of PI3K/AKT/mTOR was performed by IHC in Tissue microarray of 71 tumors and 71 normal
tissues from patients with GC. The proteins were PI3K, PTEN, AKT, phospho-AKT, mTOR, phosphomTOR, p70S6K, phospho-
p70S6K, 4E-BP1, phospho-4E-BP1, eIF4E and phospho-eIF4E. Then the expression of miR-125b and miR-451 was analyzed in 22
hispanic/amerindian GC and 22 control normal using TaqMan? miRNA assays qRT-PCR in these fresh frozen tissues.
High protein expression was found for 9 out of 12 proteins in tumor tissue compared with normal tissue (p<0.05).
Conversely, a high expression of PTEN was found in normal tissues (p<0.001). Kaplan-Meier analysis exhibited a poor survival in
those patients with low expression of 4E-BP1 (p<0.05). 00, 20, 40, 60, 81020406080100120140 Supervivencia (meses) %High Low
These data shows that PI3K/AKT/mTOR pathway is activated in tumor tissues of GC and that 4EBP1 could be a
potential prognosis biomarker in this cancer. Moreover, the downregulation of miR-125b and miR-451 suggests an important
role of these miRs in carcinogenesis probably targeting AKT and TSC1 within PI3K/AKT/mTOR pathway. These data could be
an initial approach to promote a new therapy strategy on this pathway.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals