alexa New Tools For The Follow-up Of ?- And ?-secretase Modulations And For Patients? Stratification In Alzheimer?s Disease | 22014
ISSN: 2161-0460

Journal of Alzheimers Disease & Parkinsonism
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

2nd International Conference on Alzheimers Disease and Dementia
September 23-25, 2014 Valencia Convention Centre, Spain

Frederic Checler, Celine Caillava, Sebastien Ranaldi, Lauritzen Inger, Bauer Charlotte, Ranaldi Sebastien, Robert Philippe, Fareh Jeannette and Abraham Jean-Daniel
Accepted Abstracts: J Alzheimers Dis Parkinsonism
DOI: 10.4172/2161-0460.S1.010
Abstract
Previous studies suggest the crucial role of N-terminally truncated species and endoproteolytic products of amyloid-? peptide (A?N-x and Ab34, respecively) in Alzheimer?s disease (AD). However, our knowledge about their production and levels at early stages of the disease and along its progression remains incomplete, in part due to the lack of very specific and sensitive tools for their detection. We have developed specific monoclonal antibodies that target, on one hand, the N-terminus of either A?11-x or A?17-x species, and on the other hand, the C-terminus of A?34 species. Specific multiplexed assays were set-up using different combinations of these antibodies. The specificity, sensitivity and were assessed using synthetic peptides and human cell models, before their evaluation in CSF samples from patients with AD (n=23), MCI (n=23) and controls with normal cognition (n=21). We demonstrate the high specificity of the assays, which allow the exclusive detection of the targeted peptides, without cross-reactivity towards any other tested peptides, even differing of one aminoacid. We show that the pharmacological manipulation of BACE1 or ADAM17 activity modulates both A?11-x/A?17-x and A?34 productions. Finally, A? species concentrations were evaluated in human cerebrospinal fluid (CSF). A?11-x levels were significantly lower in patients with MCI than in controls. Compared to the combination of A?1-42, T-Tau and P-Tau (AlzBio3, Innogenetics), the association of A?11-40, A?17-40 and T-Tau improved the discrimination between MCI and controls. Furthermore, when patients with MCI were classified in two subgroups (MCI?1.5 or ?2 based on their Cognitive Dementia Rating - Sum of Boxes (CDR-SB) score), the CSF A?17-40/A?11-40 ratio was significantly higher in patients with CDR-SB?1.5 than in controls, while neither A?1-42, T-Tau nor P-Tau allowed the detection of this subpopulation. Finally, Ab34 was revealed as an early biomarker of AD progression. Overall, we have documented for the first time interest of the measurement of these N-truncated peptides and A?34 in AD early diagnosis.
image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version