Journal of Gastrointestinal & Digestive System
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The diagnosis and clinical monitoring of HBV infection are based on the detection of viral antigens, antibodies to viral
proteins and circulating viral genome. The ?e? antigen is a protein secreted by hepatitis B viruses that are actively replicating
in liver cells. Some people who have had hepatitis B for many years lose the ?e? antigen, develop ?e? antibodies, but continue
to have moderately high viral load and elevated ALT levels, which indicates liver damage. Hence, though useful, serological
testing in chronic hepatitis B infection may not always give an accurate picture to the physician. The number of HBV DNA
in the blood indicates how rapidly the virus is reproducing in the liver. An accurate quantitative assay for plasma/serum HBV
DNA may monitor residual viral load during treatment and allow timely detection of flares of viral replication that accompany
the emergence of variants. In recent years, several Real-Time PCR detection assays have been used for quantification of HBV
DNA in serum or plasma. Our aim was to correlate Hepatitis B Viral loads quantified on two different Real Time PCR methods
with the immune status of HBe antigen in Chronic Hepatitis B patients. Patient samples were tested for the presence of HBeAg,
anti-HBeAb and HBV DNA. HBV DNA was quantified using Cobas? Taqman? and artus? HBV Real-Time PCR. Correlation
between the two Real Time methods (r) was found to be 0.997, regression coefficient (r2) was found to be 0.99 and correlation
significance was also high (P <0.05). 24.56% cases were HBeAg-negative but had HBV DNA detected. Fifty per cent of these cases
had undergone HBeAg seroconversion. The study and the use of different Real Time PCR systems for HBV viral load estimation
will be discussed during the talk.
Arnab Roy obtained his MBBS from Calcutta Medical College and MD in Clinical Biochemistry & Genetics from Institute of Medical Sciences (IMS),
Banaras Hindu University, India. Currently he works as a senior research scientist at SRL Ltd in its R&D division and in the additional capacity of
knowledge management lead. His area of focus is molecular diagnostics with emphasis on viral load testing and metabolic genetics. He has more
than 5 publications to his credit in his field of expertise.
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