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Prevalence and resistance to antibiotics of Enterobacteriaceae and non-fermentative bacilli isolated at the military hospital specialized in orthopedics at Algiers (2009-2014)

World Congress on Infectious Diseases

Aouf Abdelhakim1, RasledjbelYoucef2 and Bakour Rabah1

Posters-Accepted Abstracts: J Infect Dis Ther

DOI: 10.4172/2332-0877.S1.003

Abstract

The medical community relies on clinical expertise and published guidelines to assist physicians with choices in empirical
therapy. ESBL-Enterobacteriaceae and non-fermentative bacilli resistant to carbapenems have emerged within the
community setting as an important cause of a variety of infections. The aim of this study is to determine the prevalence of
these pathogens, to evaluate their level of resistance, the phenotypic characterization of resistance to carbapenems and 3GC
and the possibility of horizontal transfer. Also to testByotrol (disinfectant) on selected multi-drug resistant isolates. This work
was realized at orthopedic, reeducation and reanimation services on hospitalized and non-hospitalized patients.On a total of
1482 positive samples, Enterobacteriaceae were the predominant (44%), followed by Staphylococcus sp. (37%), Pseudomonas
aeruginosa (13%) and Acinetobacter baumannii (4%). Even the low rate of isolation of A.baumannii it was responsible of
high mortality rate (9%). The most isolated species of Enterobacteriaceae were E. coli, Klebsiella pneumoniae, Enterobacter
cloacae, Proteus mirabilis and Serratia marsescens. Susceptibility to antibiotics showed that isolates have acquired high level
of resistance. It concerns nearly all antibiotic families used in therapy. E. cloacae was the most ESBL producer followed by
K. pneumoniae and E. coli. ESBLs are plasmid mediated in association with other antibiotics. Resistance of A. baumannii
to carbapenems was almost due to production of metallo-β-lactamases, and the lost D2 porines. All selected MDR isolates
were sensitive to disinfectant tested and colistin. The judicious choice and use of antibiotics and disinfectants may reduce
consequently the dissemination of multi-drug resistant clones.

Biography

Aouf Abdelhakim received his Ph.D. degree in Microbiology at Ain Shams university-Cairo (2007), the work was advised by Pr. Bakour Rabah, Pr. Mohamed Sayed
Salama and Dr. Hala abouchady, and had participated in two projects at the laboratory of cellular and molecular biology (Faculty of biology-University of sciences
and technologie Houari Boumediene) about resistance to antibiotics of Gram negative bacilli. His primary field is Medical and molecular bacteriology with research
emphasis on resitance of Enterobacteriaceae and non-fermentative bacilli to antibiotics and disinfectants.

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