Antioxidant Assessment of Pleurotus sajor-caju Extracts Obtained through Hot Extraction Process
Current work reports antioxidant activities of extracts derived from P. sajor-caju using several in vitro models. Dried samples were sequentially extracted in hexane, ethyl acetate, methanol and water using hot extraction method. Extraction with hexane and ethyl acetate resulted in negligible recovery. Methanol and water extracts tested positive for phenolics, flavonoids, terpenoids, anthraquinones and saponins. Total phenol and flavonoid contents were found in the range of 19.78-23.59 μg propyl gallate equivalent/mg and 23.27-26.44 μg quercetin equivalent/mg in aqueous and methanolic extracts, respectively. Comparatively lower activities were observed for both the extracts in DPPH radical scavenging and reducing power assays. Appreciable metal ion chelating activity was demonstrated by aqueous (57% to 86%) and methanolic (36% to 71%) extracts in the concentration range 100-400 μg/ml, showing dose dependent response. Total antioxidant capacity for both the extracts was found in the range 83-96 μg PGE/ml at test concentration. The study showed P. sajor-caju aqueous and methanolic extracts have antioxidant potential.