alexa

GET THE APP

Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
Anti-Prostate Specific Antigen (Anti-PSA) Modified Interdigitated Microelectrode-Based Impedimetric Biosensor for PSA Detection | OMICS International | Abstract
ISSN: 2090-4967

Biosensors Journal
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Anti-Prostate Specific Antigen (Anti-PSA) Modified Interdigitated Microelectrode-Based Impedimetric Biosensor for PSA Detection

Sunil K. Arya* and Shekhar Bhansali

Bio-MEMS and Microsystems Laboratory, Department of Electrical Engineering, University of South Florida, 4202 E. Fowler Avenue, ENB 118, Tampa, FL 33620, USA

*Corresponding Author:
Sunil K. Arya
E-mail: [email protected]

Received date: 9 June 2011; Accepted date: 23 August 2011; Published date: 24 August 2011

Abstract

Interdigitated microelectrodes (IDEs) functionalized with a cysteamine (Cys) self-assembled monolayer (SAM) were used to fabricate a sensitive, disposable, impedimetric biosensor for prostate specific antigen (PSA) detection. PSA specific monoclonal antibody (anti-PSA) was covalently immobilized on cysteamine modified gold surface of IDEs, using N-ethyl-N’-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide (EDC/NHS) chemistry. Bovine serum albumin (BSA) was used as a blocker to prevent nonspecific adsorption on electrode surface. BSA-anti-PSA/Cys/IDE electrodes were exposed to solutions with different PSA concentrations and their response was measured using a label-free electrochemical impedance (EIS) technique. The impedance changes revealed close a correlation with PSA concentrations in the measurement range of 1 pg.mL−1–100 ng.mL−1, with sensitivity of 0.444 mL.g−1.

Keywords

Top