Effects of Naphthalene Acetic Acid (NAA) and Indole -3- Butyric Acid (IBA) on In Vitro Rooting of Sugarcane (Saccharum officinarum L.) Micro- ShootsBelay Tolera*
Ethiopian Sugar Corporation, Research and Training Division, Biotechnology Research Team, Wonji Research Center, P.O. Box 15, Wonji, Ethiopia
- Corresponding Author:
- Belay Toler
Ethiopian Sugar Corporation
Research and Training Division
Variety Development Directorate
Biotechnology Research Team
Wonji Research Center
P.O. Box 15, Wonji, Ethiopia
E-mail: [email protected]
Received date: September 17, 2015; Accepted date: December 28, 2015; Published date: January 04, 2016
Citation: Tolera B (2016) Effects of Naphthalene Acetic Acid (NAA) and Indole -3- Butyric Acid (IBA) on In Vitro Rooting of Sugarcane (Saccharum officinarum L.) Micro-Shoots. J Biotechnol Biomater 6:215. doi:10.4172/2155-952X.1000215
Copyright: © 2016 Tolera B. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In Vitro rooting of micropropagated micro-shoots of two commercial sugarcane varieties was carried out with the aim of evaluating the root induction responses of the sugarcane varieties (B41-227 and N14) to alpha naphthalene acetic acid (NAA) and Indole -3- butyric acid (IBA). Accordingly, four levels of NAA (0, 1, 2, and 3 mg L-1) and IBA (0, 1, 2 and 3 mg L-1) in a completely randomized design with 4 × 4 × 2 factorial treatment combination arrangements were tested. Data on the number of roots per shoot and average root length (cm) were collected after 30 days of culture on ½ MS root induction medium. Analysis of variance revealed that the interaction effects of NAA, IBA and the sugarcane genotypes on number of roots per shoot and average root length of both sugarcane varieties was very highly significant (P<0.0001). Culture medium containing 2 mg L-1 NAA and 1 mg L-1 IBA for B41-227 and 1 mg L-1 NAA alone for N14 was found to be optimum. On these medium, B41-227 gave 33 ± 0.15 roots per shoot with 2.92 ± 0.18 cm root length and N14 produced 35± 0.20 roots per shoot with 3.2 ± 0.25 cm root length. The rooted plantlets were survived 100% after four weeks of acclimatization in greenhouse on Farmyard manure and soil at 2:8 ratios. The optimized protocol can be used to develop healthy and profuse root system in the sugarcane micro-shoots, an essential stage in sugarcane micropropagation.