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Hindi Research Article
Genomic Analysis of Mycobacterium tuberculosis in Respiratory Positive- Smear Patients Using PGRS-RFLP in Northwest and West Provinces of Iran
| Bahram Golestani Eimani1* and Maryam Seyyedi2 | |
| 1Molecular Genetic, Department of Biology, Urmia Azad University, urmia, Iran | |
| 2Tuberculosis and Lung Disease Research Center, Tabriz University of Medical Sciences, Tabriz, Iran | |
| Corresponding Author : | Bahram Golestani Eimani Department of Biology, Urmia Azad University, urmia, Iran Tel: 441-262-0124 E-mail: golestani_bahram@yahoo.com |
| Received: November 18, 2015 Accepted: December 17, 2015 Published: December 21, 2015 | |
| Citation: Eimani BG, Seyyedi M (2015) Genomic Analysis of Mycobacterium tuberculosis in Respiratory Positive- Smear Patients Using PGRS-RFLP in Northwest and West Provinces of Iran. J Obes Weight Loss Ther 5:289. doi:10.4172/2165-7904.1000289 | |
| Copyright: © 2015 Eimani BG, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
| Related article at Pubmed, Scholar Google | |
Abstract
Determining and clustering of Mycobacterium tuberculosis strains is of great application in control programs of tuberculosis. Identification of transmission type and tracking the infection source is also highly necessary. The present study was performed aiming to track and determine the type of Mycobacterium tuberculosis infection, as well as its relationship with demographic factors, using PGRS-RFLP. Materials and methods: In this cross-sectional study, 84 smear-positive patients from 5 frontier provinces (East Azerbaijan, West Azerbaijan, Ardebil, Kurdistan, and Kermanshah) were investigated according to PGRS-RFLP. Demographic data were collected using a questionnaire. The results were analyzed by SPSS-18 and G-Box. Findings: Based on clustering, recent transmission was 66%. Most clusters were obtained from Kurdistan and Kermanshah. Vaccination record (p=0.49) and treatment group (without previous treatment) (p=0.004) had a significant relationship with clustering. Other demographic factors including age, gender, religion, drug abuse, smoking, history of migration, and marital status did not show a significant relationship with clustering. Conclusion: Genetic variation of Mycobacterium tuberculosis is high in this region. The rate of recent transmission based on clustering was unexpected (global average is 30-40%). Recent transmission was more dynamic in the west than the northwest Iran. The strong relationship between the treatment group 1 (without previous treatment) and clustering based on PGRS-RFLP can demonstrate the high correlation between molecular and classic information. In addition, the significant relationship between vaccination record and clustering highlights the necessity to conduct more extensive studies.
