Journal of Community Medicine & Health Education
Open Access

Imaging and SERS Study of the Au Nanoparticles Interaction with HPV and Carcinogenic Cervical Tissues

Andrea Ceja-Fdez, Ramón Carriles, Ana Lilia González-Yebra and Tzarara López-Luke^*

¹Division of Sciences and Engineering Campus León, Department of Medical Physics, University of Guanajuato, Mexico

²Centro de Investigaciones en Óptica, Mexico

³Edificio U, Ciudad Universitaria, Morelia, Institute for Research in Metallurgy and Materials, Universidad Michoacana de San Nicolás de Hidalgo, Mexico

⁴Health Sciences Division, Department of Medicine and Nutrition, University of Guanajuato, Campus Le�n, Mexico

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In this work, gold NPs were prepared by Turkevich method and their interaction with the HPV and cancerous cervical tissues by scanning electron microscopy, energy dispersive x-ray spectroscopy, confocal and multiphoton microscopy and SERS were studied. The SEM images confirmed the presence and localization of the gold NPs inside of the two kind of tissues. The absorption light of the gold NPs was at 520 nm. However, it was possible to obtain two-photon imaging (red emission region) of the gold NPs inside of the tissue, exciting the samples at 900 nm, observing the morphology of the tissues. The infrared absorption was due probably by the aggregation of gold NPs inside of the tissues. Therefore, the interaction of gold nanoparticles with the HPV and cancerous cervical tissues a surface enhanced Raman spectroscopy (SERS) was obtained. As preliminary studies, having an average of 1000 Raman spectra per tissue, SERS signals showed changes between the HPV infected and the carcinogenic tissues; these spectral signatures occurred mainly in the DNA bands, potentially offering a tool for rapid screening of cancer.

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