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1165-158X

Cellular and Molecular Biology
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Research Article

Isolation and In vitro Validation of Cardiac Muscle-Specific Promoters in Pigs

Sangsu Shin1,2#, Jinsoo Ahn1,3#, Yeunsu Suh1, Steven J. Moeller1, Seongsoo Hwang1, 4* and Kichoon Lee1,3*

1Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA

2Department of Animal Biotechnology, Kyungpook National University, Sangju, Gyeongbuk 37224, Republic of Korea

3The Ohio State University Interdisciplinary Ph.D. Program in Nutrition, The Ohio State University, Columbus, OH 43210, USA

4Animal Biotechnology Division, National Institute of Animal Science, RDA, Wanju-gun, Jeonbuk 55365, Republic of Korea

#Equally Contributed

*Corresponding Author:
Seongsoo Hwang
Animal Biotechnology Division, National
Institute of Animal Science, Republic of Korea
Tel: +82-63-238-7253
Fax: +82-63-238-7297
E-mail: [email protected]
Kichoon Lee
Department of Animal Sciences
The Ohio State University, Columbus, OH 43210, USA
Tel: 614-688-7963
Fax: 614-292-2929
E-mail: l[email protected]

Received date: April 23, 2016; Accepted date: May 10, 2016; Published date: May 15, 2016

Citation: Shin S, Ahn J, Suh Y, Moeller SJ, Hwang S, et al. (2016) Isolation and In vitro Validation of Cardiac Muscle-Specific Promoters in Pigs. Cell Mol Biol 62:123. doi: 10.4172/1165-158X.1000123

Copyright: © 2016 Shin S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Identification of promoter sequences that can drive heart-specific expression of transgenes is essential to investigate gene function in the heart. The aim of this study was to identify heart-specific genes and their promoter sequences that can promote heart-specific transgene expression in pig primary heart cells. Gene expression profiles in the Gene Expression Omnibus (GEO) repository have been integrated and utilized to identify MYH6, TNNI3, and MYBPC3 as common heart-specific genes in the mouse and human. RT-PCR further confirmed heartspecific expression of the genes among various tissues in pigs as well as in mice and humans. Bioinformatics analysis predicted that their promoter sequences contain multiple binding sites for transcription factors involved in cardiogenesis and the promoter sequences were substantially conserved between pigs and humans. In addition, in vitro analysis showed that expression of (i.e., the green fluorescence protein (GFP)) reporter gene under the regulation of promoter sequences of MYH6, TNNI3, and MYBPC3 was detected in primary pig heart cells but not in other primary cells. These findings, along with the physiologic similarities between humans and pigs, suggest that these novel promoters may be valuable candidates for the regulation of heart gene expression in both humans and pigs for biomedical purposes.

Keywords

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