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Isolation and In vitro Validation of Cardiac Muscle-Specific Promoters in
Sangsu Shin1,2#, Jinsoo Ahn1,3#, Yeunsu Suh1, Steven J. Moeller1, Seongsoo Hwang1, 4* and Kichoon Lee1,3*
1Department of Animal Sciences, The Ohio State University, Columbus, OH 43210, USA
2Department of Animal Biotechnology, Kyungpook National University, Sangju, Gyeongbuk 37224, Republic of Korea
3The Ohio State University Interdisciplinary Ph.D. Program in Nutrition, The Ohio State University, Columbus, OH 43210, USA
4Animal Biotechnology Division, National Institute of Animal Science, RDA, Wanju-gun, Jeonbuk 55365, Republic of Korea
Animal Biotechnology Division, National
Institute of Animal Science, Republic of Korea Tel: +82-63-238-7253 Fax: +82-63-238-7297 E-mail:[email protected]
Department of Animal Sciences
The Ohio State University, Columbus, OH 43210, USA Tel: 614-688-7963 Fax: 614-292-2929 E-mail: l[email protected]
Received date: April 23, 2016; Accepted date: May 10, 2016; Published date: May 15, 2016
Citation: Shin S, Ahn J, Suh Y, Moeller SJ, Hwang S, et al. (2016) Isolation and In vitro Validation of Cardiac Muscle-Specific Promoters in Pigs. Cell Mol Biol 62:123. doi: 10.4172/1165-158X.1000123
Identification of promoter sequences that can drive heart-specific expression of transgenes is essential to investigate gene function in the heart. The aim of this study was to identify heart-specific genes and their promoter sequences that can promote heart-specific transgene expression in pig primary heart cells. Gene expression profiles in the Gene Expression Omnibus (GEO) repository have been integrated and utilized to identify MYH6, TNNI3, and MYBPC3 as common heart-specific genes in the mouse and human. RT-PCR further confirmed heartspecific expression of the genes among various tissues in pigs as well as in mice and humans. Bioinformatics analysis predicted that their promoter sequences contain multiple binding sites for transcription factors involved in cardiogenesis and the promoter sequences were substantially conserved between pigs and humans. In addition, in vitro analysis showed that expression of (i.e., the green fluorescence protein (GFP)) reporter gene under the regulation of promoter sequences of MYH6, TNNI3, and MYBPC3 was detected in primary pig heart cells but not in other primary cells. These findings, along with the physiologic similarities between humans and pigs, suggest that these novel promoters may be valuable candidates for the regulation of heart gene expression in both humans and pigs for biomedical purposes.