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Hindi MD2 mediates inflammation and nerve repair in sciatic nerve injury
*Corresponding Author:Received Date: Feb 02, 2024 / Published Date: Mar 18, 2025
Citation: Xu D, Li S, Li W, Ding J, Chen T (2025) MD2 Mediates Inflammation and Nerve Repair in Sciatic Nerve Injury. Diagnos Pathol Open 10: 248.
Copyright: © 2025 Xu D, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
Abstract
When the peripheral nerve is physically injured, activated Schwann cells and recruited macrophages release a variety of inflammatory cytokines, such as TNFα, IL-1β, IL-6. Myeloid Differentiation protein 2 (MD2) is a secreted protein composed of 160 amino acids, it is a valuable anti-inflammatory target and can be utilized in a number of inflammatory contexts. However, the role of MD2 in peripheral nerves is unclear. In this experiment, our aim was to study whether MD2 is involved in the inflammatory response of peripheral nerve injury and its role in the repair of peripheral nerve injury. We established a model of sciatic nerve injury, real-time quantitative PCR, immunoblotting and immunofluorescence staining showed that MD2 was upregulated after sciatic nerve injury and the inflammatory reaction in MD2 Knockout (MD2-KO) mice against C57BL/6 background was lighter than that in C57BL/6 mice. The results of immunoblotting and immunohistochemical staining indicated that Growth Associated Protein 43 (GAP43) and p75 Neurotrophic Factor Receptor (p75NTR) were highly expressed in MD2-KO mice after sciatic nerve injury. Enhanced sciatic function index in MD2-KO mice suggested that knockout of MD2 was beneficial to the repair of sciatic nerve injury. In summary, we conclude that MD2 participates in the inflammatory reaction of peripheral nerve injury and plays an important role in the repair of peripheral nerve injury.
