Mesoglein Expression During Aurelia aurita Life CycleAnastasiya Kotova1, Anastasiya Naiden1, Alexander Shumeev1, Tatiana Shaposhnikova1, Olga Podgornaya1 and Leonid Adonin2*
- *Corresponding Author:
- Leonid Adonin
Institute of Cytology RAS, St.-Petersburg
Russia; Far Eastern Federal University
School of Biomedicine, Vladivostok
Tel: 007 911155-4469
E-mail: [email protected]
Received date: November 01, 2016; Accepted date: November 15, 2016; Published date: November 25, 2016
Citation: Kotova A, Naiden A, Shumeev A, Shaposhnikova T, Podgornaya O, et al. (2016) Mesoglein Expression During Aurelia aurita Life Cycle. J Marine Sci Res Dev 6: 211. doi: 10.4172/2155-9910.1000211
Copyright: © 2016 Kotova A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Cnidarian is thought to possess two tissue layers: endoderm (gastroderm) and ectoderm, which are separated by the layer of extracellular matrix (ECM) called mesoglea. Aurelia aurita complex life cycle consists of several stages including alternating generations of sexual adult stage medusa and asexual stage polyp (scyphistoma). The main difference between polyp and medusa is the degree of the ECM (mesoglea) development. The new protein “mesoglein” was determined as one of the main components of mesoglea. Mesoglein is the component of the mesoglea “elastic” fibers. Previously we found that according to reverse transcription PCR mesoglein is expressed in the mature medusa exclusively in the mesogleal cells. The aim of the current work was to find out at what stage of development the specific mesoglein expression occurs. Mesogleins’ expression have been checked by PCR with specific primers on the template of transcriptomes’ cDNA from different stages; by mesogleins’ antibody staining on SDSPAGE and on paraffin sections; by histochemistry staining. In A.aurita life cycle mesoglein synthesis begins at scyphystoma polyp stage at RNA and protein level in mesogleal cells (Mc) which separated at this stage as the cell type. Mesoglein stored in granules both in Mc of adult medusa and polyp on its’ way to ECM elastic fibers. Our data perfectly correspond to the recently reported de novo transcriptome assembled from Illumina RNA-Seq data generated from six stages throughout the Aurelia life cycle.