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Preliminary Study of the Identification of Proteins in Tissue Fluids of the Sea Mussel Isognomon alatus | OMICS International | Abstract
ISSN: 2155-9910

Journal of Marine Science: Research & Development
Open Access

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Short Communication

Preliminary Study of the Identification of Proteins in Tissue Fluids of the Sea Mussel Isognomon alatus

Angel Alberto Justiz Vaillant1*, McFarlane-Anderson2, Monica Smikle3, Brian Wisdom4, Wayne Mohammed1, Sehlule Vuma1, Geeta Kurhade5, AV Chalapathi Rao1 and Arvind Kurhade1

1Pathology and Microbiology Unit, Department of Para-Clinical Sciences, University of the West Indies, St. Augustine, Trinidad and Tobago, West Indies

2Department of Basic Medical Sciences, University of the West Indies, Mona, Jamaica, West Indies

3Department of Microbiology, University Hospital of West Indies, Mona, Jamaica, West Indies

4School of Biology and Biochemistry, Medical Biology Centre, The Queen’s University of Belfast, UK

5Physiology Section, Department of Pre-clinical Sciences, University of the West Indies, St Augustine, Trinidad and Tobago, West Indies

*Corresponding Author:
Angel Justiz Vaillant
Department of Para-Clinical Sciences
The University of the West Indies
St. Augustine, Trinidad & Tobago, West Indies
Tel: +868-736-0440
Fax: +868-663-3797
E-mail: [email protected]

Received date: September 14, 2013; Accepted date: January 31, 2014; Published date: February 10, 2014

Citation: Vaillant AAJ, Farlane-Anderson M, Smikle M, Wisdom B, Mohammed W, et al. (2014) Preliminary Study of the Identification of Proteins in Tissue Fluids of the Sea Mussel Isognomon alatus. J Marine Sci Res Dev 4:147. doi:10.4172/2155-9910.1000147

Copyright: © 2014 Vaillant AAJ. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The aim of this preliminary study was to investigate the presence of proteins in the tissue fluids of the sea
mussel
Isognomon alatus. Protein extraction was done by the chloroform-cold ethanol technique. Immunization for
production of antibody to be used as reagents in Western blotting, assessment of the protein concentration by the
Bradford method, protein characterization by native polyacrylamide gel electrophoresis (PAGE) were also performed
as a part of the methodology of this study. The results showed a protein content of 65 mg/ml in tissue fluids and a
protein band approximately of 220 kDa in PAGE that was further confirmed by the Western blotting. Future work
should investigate the structure and function of the proteins separated from the tissue fluids and we considered it
as a limitation of this investigation. The sea bivalve literature is scanty. However the limitation of this work we still
can conclude that there are high molecular weight proteins in large concentrations in tissue fluids of the sea mussel
Isognomom alatus.

Keywords

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