ISSN: 2161-0711

Journal of Community Medicine & Health Education
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Research Article

Prevalence of Cryptosporidium in Children with Diarrhea in North Indian Tertiary Care Hospital

Farhat Tahira1*, Haris M. Khan1, Indu Shukla1, Fatima Shujatullah1*, M. Ashraf Malik2 and M. Shahid1

1Department of Microbiology, J.N. Medical College, Aligarh Muslim University, Aligarh, UP, India

2Department of Pediatrics, J.N. Medical College, Aligarh Muslim University, Aligarh, UP, India

Corresponding Author:
Fatima Shujatullah
Department of Microbiology
J.N. Medical College, Aligarh Muslim University
Aligarh, UP, India
Tel: +919837568948
E-mail: sfatima777@gmail.com, farhat_thr@yahoo.co.in

Received Date: March 15, 2012; Accepted Date: March 29, 2012; Published Date: March 31, 2012

Citation: Tahira F, Khan HM, Shukla I, Shujatullah F, Malik MA, et al. (2012) Prevalence of Cryptosporidium in Children with Diarrhoea in North Indian Tertiary Care Hospital. J Community Med Health Edu 2:136. doi:10.4172/jcmhe.1000136

Copyright: © 2012 Tahira F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Background: Enteric parasitic protozoan of genus cryptosporidium has become an important cause of diarrhoea in developing countries. It is a cause of acute and persistent diarrhoea in immunocompromised and immunocompetent subjects.

Methodology: This study was conducted to determine the prevalence of Cryptosporidium spp. as an enteric pathogen causing diarrhoea in children. 250 stool specimens of children with diarrhoea were examined using staining procedures like modified Ziehl-Neelsen (Z.N.) staining, safranine-methylene blue staining after concentration of stool specimens by formol-ether concentration technique and antigen detection ELISA was performed for diagnosis.

Results: Modified Z. N. staining and safranine-methylene blue staining were positive in 21 cases while 29 cases were positive by ELISA. Prevalence of Cryptosporidium in children with diarrhoea was found to be 11.6%.

Conclusion: High prevalence as noted in this study advocates need to screen for this parasite as a routine diagnostic measure in patients of diarrhoea.

Keywords

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