SCREENING OF ANTIBACTERIAL ACTIVITY, QUALITATIVE AND QUANTITATIVE ANALYSIS OF PHYTOCHEMICALS IN VITEX TRIFOLIA
The present focus of the study was to evaluate the antibacterial activity and phytochemical analysis in the medically important plant vitex trifolia leaf extract by using solvents like aqueous, methanol, acetone, benzene, petroleum ether. The plant vitex trifolia used for its phytochemical and pharmacological studies. In qualitative and quantitative analysis of vitex trifolia viz., alkaloids, saponin, tannin, phenols, terpenoids, flavonoids, steroids, were determined using standard methods. Vitex trifolia leaves were collected, air-dried and soxhlet extracted by using standard method. These extracts were then tested for antimicrobial activity using disc diffusion method. Vitex trifolia showed highest antibacterial activity (IZ= 0.5cm in acetone extract, IZ = 0.75 in ethanol extract) against Bacillus subtilis and (IZ= 0.15cm in acetone extract, IZ = 0.25cm in ethanol extract, IZ = 0.35cm in aqueous extract) against Escherichia coli. Results concluded that the phytochemicals of leaves extract of vitex trifolia has the potential to act as a antibacterial agent.