SCREENING OF ECLIPTA ALBA EXTRACTS FOR ANTICANCER ACTIVITY
Sleep deprivation can lead to various alterations at physiological and psychological levels such as EEG changes, metabolic changes, irritation, blurred vision, memory lapses, hallucinations, psychosis and can even lead to death. Treatment still remains a challenge as hypnotics are associated with side effects. The objective of the present study was to investigate the protective effects of melatonin and buspirone and their combinations against the biochemical and behavioral alterations induced by 48 hrs sleep deprivation in mice. Pretreatment with melatonin (2.5 mg/kg and 5 mg/kg), buspirone (5 mg/kg and 10 mg/kg), and melatonin (2.5, 5 mg/kg) in combination with buspirone (5 mg/kg) showed significant improvement in behavioral parameters such as increase in body weight, increase in locomotor activity, and reduction in anxiety like behavior. Biochemical parameters estimation also revealed similar results such as significant attenuation of lipid peroxidation and nitrite concentration and significant elevation of glutathione and catalase levels following treatment with melatonin (2.5, 5 mg/kg) and buspirone (5,10 mg/kg) and melatonin (2.5, 5 mg/kg) in combination with buspirone (5 mg/kg) as compared to their effect per se. Thus, preliminary findings suggest the protective effect of melatonin and buspirone and their combinations against sleep deprivation and associated alterations.