SIMULTANEOUS ESTIMATION OF CLOTRIMAZOLE AND TINIDAZOLE IN A TABLET USING REVERSE PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
A Simple, efficient and reproducible method for the simultaneous determination of Clotrimazole and Tinidazole from pharmaceutical formulation has been developed using reversed phase high performance liquid chromatography. The separation was carried out using a mobile phase consisting of methanol :
acetonitrile (95:5 % V/V). Column used was chromacil C18 (250 X 4 mm internal diameter) 5 μ with flow rate of 1 ml/min. The detection wavelength used was 229nm. The mean retention time of Clotrimazole and Tinidazole were 6.84 min and 5.54 min respectively. Linearity of both drugs are 10-50 μg/ml. Analytical
parameters were calculated and a full statistical evaluation included.