alexa The Establishment of Biosensor Technology Based on F0F1-ATPase Molecular Motor for Detection of Rotavirus and Hepatitis A Virus
ISSN: 2090-4967

Biosensors Journal
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

The Establishment of Biosensor Technology Based on F0F1-ATPase Molecular Motor for Detection of Rotavirus and Hepatitis A Virus

Jie Zhang1*, Zhuo Zhao2, Mei-Ling Xu3, Xiang-Ying Yang1 and Zhi-Peng Liu2

1Beijing Inspection and Quarantine Testing Center, Beijing Entry-Exit Inspection Quarantine Bureau, Beijing, China

2Technical Center for Safety of Industrial Products, Tianjin Entry-Exit Inspection Quarantine Bureau, Tianjin, China

3Linyi Entry-Exit Inspection Quarantine Bureau, Linyi, China

Corresponding Author:
Zhang J, Beijing chaoyang
TianShuiYuan street no. 6, Beijing Inspection And Quarantine Testing Center
Beijing Entry-Exit Inspection Quarantine Bureau, Beijing, China, 100026
Tel/Fax: +86-10-58619231

Received Date: May 29, 2015 Accepted Date: August 18, 2015 Published Date: August 24, 2015

Citation: Zhang J, Zhao Z, Xu M, Yang X, Liu Z (2015) The Establishment of Biosensor Technology Based on F0F1-ATPase Molecular Motor for Detection of Rotavirus and Hepatitis A Virus. Biosens J 4:121. doi:10.4172/2090-4967.1000121

Copyright: © 2015 Zhang J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Around the world, food-borne virus is one of the main pathogenic microorganisms in the aspect of causing people and animals with acute diarrhoea and poses a serious threat to human health. Therefore, the rapid detection of foodborne virus is very important to guarantee food safety and human health. Here, we reported that we developed a specific, convenient and fast detected method to detect rotavirus (RV) and hepatitis A virus (HAV) by using F0F1-ATPase molecular motor biosensor. Specific RNA probes were encompassed the conservative region of food-borne virus, and a molecular motor detect device was constructed by connecting probes to F0F1-ATPase molecular motor through biotin-streptavidin system. Extracted virus RNA was conjugated with the biosensor separately and meanwhile ATP was synthesized. By comparing their fluorescence intensity, virus RNA level was detected. Our results demonstrated that this biosensor’s sensitivity was the concentrations of 0.005 ng/mL and 0.01 ng/mL for RV and HAV respectively. Furthermore, this method possessed specificity for RV and HAV and none cross-reaction between them. What’s more, this method could be accomplished within 1h. We detected 15 samples by using this method and the results were consistent with RT-PCR results. Overall, this new-typed method based on F0F1-ATPase molecular motor biosensor for RV and HAV detection is sensitive and specific and can be used in the rapid detection of food-borne virus.


Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

1-702-714-7001Extn: 9037

Business & Management Journals


1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version