The Study of Tyrosinase and Antioxidant Activity of Xylocarpus Granatum Koenig Seed Kernel Extract toward Evidence Based Indigenous Knowledge from Togean Archipelago, IndonesiaZamani NP1*, Gazali M2 and Batubara I3
- *Corresponding Author:
- Zamani NP
Bogor Agricultural University
Faculty of Fisheries and Marine Science
Kampus IPB Dramaga, West Java, Indonesia
Tel: + 622518629131
Fax: + 622518626380
E-mail: [email protected]
Received date: August 20, 2015; Accepted date: October 16, 2015; Published date: October 22, 2015
Citation: Zamani NP, Gazali M, Batubara I (2015) The Study of Tyrosinase and Antioxidant Activity of Xylocarpus granatum Koenig Seed Kernel Extract toward Evidence Based Indigenous Knowledge from Togean Archipelago, Indonesia. J Marine Sci Res Dev 5:168. doi:10.4172/2155-9910.1000168
Copyright: © 2015 Zamani NP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The tyrosinase inhibitory activity and antioxidant activity of three (3) extracts (methanol, chloroform,and n-hexane) from seed kernel of Xylocarpus granatum Koenig was tested. The tyrosinase inhibitory activity and antioxidant activity was estimated as IC50 values and the content of total phenolics in extracts was calculated as gallic acid equivalent (GAE). The best result of tyrosinase inhibitory ability was obtained in methanol extract in monophenolase activity (IC50 =323.11 ± 9.0 μg/mL that correlated significantly with the total of phenolic content (R2 =0.98, Y= -12.27x + 471.73) and the antioxidant activity have IC50 = 10.61 ± 0.6 μg/mL. We indicate that this methanolic extract of X. granatum seed kernel posseses potency as tyrosinase inhibitor and antioxidant agent. This result has already shown that the indigenous knowledge in utilization of X. granatum seed kernel for lightening skin has been evident.