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In Association with
Analysis of microbial communities from oil contaminated sites by NGS sequencing
6th World Congress on Biotechnology
Abhisek Dasgupta, Rajal Debnath, Megha Goswami, Priyanaka Dutta and Ratul Saikia
CSIR-North East Institute of Science and Technology, India
Posters-Accepted Abstracts: J Biotechnol Biomater
Abstract
In the last few decades there has been a giant leap in the DNA sequencing technique from capillary method to high-throughput sequencing. The main advantage of high-throughput sequencing over capillary method is that the former uses shot-gun approach, therefore eliminating the tedious task of constructing clone libraries and also it uses parallel sequencing approach. In this study we used high-throughput sequencing for bacterial communities residing in oil contaminated region of Assam, India. Illumina MiSeq platform was used for generating 16S rDNA hyper variable V3 region reads. These sequences were further processed using QIIME software and other bio-informatics tool. Lastly we concluded our study by comparing species richness within and in-between samples using statistical method.Biography
Email: abhisekbiotech@gmail.com
