alexa
Reach Us +441474556909
Editor - Rushikesh V Sable | National Cancer Institute | 24783

Journal of Cell Science and Apoptosis
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Rushikesh V Sable

Rushikesh V Sable
Rushikesh V Sable
Postdoctoral Cancer Research
National Cancer Institute (NCI)
E-mail: [email protected]

Biography

Experienced in mouse bone marrow macrophages extraction, polarization, tissue digestion to separate immune cells. • Experienced in design, synthesis and bioanalytical characterization of small peptide/peptidomimetic molecules for protein-protein interactions inhibition between co-stimulatory molecules for immunomodulation which have significance in autoimmune diseases like Rheumatoid Arthritis. Worked on grafting active peptide sequence onto multicyclic stable peptide (Cyclotide) frameworks. • Expertise in cell culture and several in-vitro cells-based model assays which includes human T lymphocytes, synoviocyte cells from rheumatoid arthritis patient (HFLS-RA), and several cancer cell lines. • Worked on the study of molecular mechanism of immunomodulation by evaluating T-cell signaling by western blot analysis. • Expertise in bioanalytical methods including, Flow Cytometry, RTPCR, Confocal microscopy, HPLC, Surface Plasmon Resonance (SPR), Circular Dichroism, and Mass Spectrometry. • Experienced in the evaluation of protein-protein interaction by Proximity Ligation assay (PLA), Cell adhesion assay and E-rosette assay. • Worked with collaborators on transgenic animal models (ex-vivo) to study the T-cell proliferation and immunogenicity of small peptide molecules in case of Rheumatoid arthritis. • Developed Thermal, Chemical and Human serum stability methods for the peptide/peptidomimetic and peptide-drug conjugate molecules. • Worked on S2 insect cell transfection and protein purification using ultrafiltration, ion exchange chromatography, dialysis of proteins and detection of proteins by HPLC/FPLC analysis. • Expertise in computational methods for docking and dynamics for small ligand molecules (Autodock tools 4.1 and Discovery Studio 4.0). • Experienced in basic statistical data analysis by SAS software.

Research Interest

2017 – Present: Cancer Research Training Award Postdoctoral Fellow at National Cancer Institute (NCI): Peptide based Cancer Immunotherapy involving M2- Tumor Associated Macrophages (TAM) for pancreatic cancer. 2013 – 2016: Research/Teaching Assistant at University of Louisiana at Monroe, LA (Ph.D.): Designing of peptidomimetics to modulate autoimmune response by inhibiting costimulatory molecules protein-protein interaction (CD2-CD58) in the progression of Rheumatoid Arthritis. I worked on grafting of cyclotide framework based peptide drugs, which were found to be more stable than normal peptides. It includes design, synthesis, in-vitro cellular assays, stability testing and in-vivo studies. 2015 - Summer Research Internship program 2015 Louisiana State University (LSU) at Baton Rouge LA. (Internship): Design and synthesis of fluorescent conjugates of grafted peptides to study the molecular mechanism behind their immunomodulation property in case of autoimmune diseases

Top