|Biochemistry Department, Cellular and Molecular Research Center, Razi Drug Research Center, Tehran University of Medical Sciences, Tehran, Iran
|*Corresponding Author :
Cellular and Molecular Research Center, Razi Drug Research Center
Tehran University of Medical Sciences, Tehran, Iran
|Received November 16, 2012; Accepted November 19, 2012; Published November 21, 2012
|Citation: Najafi M (2012) Bioinformatics Tools as Power Hypothetical Predictors. Biochem Physiol 1:e113. doi:10.4172/2168-9652.1000e113
|Copyright: © 2012 Najafi M. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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|With the development and completion of genome projects, the genome data not only are available on the search of primary databases, but also the structural and functional prediction powers are increased on the growth of secondary databases. Although, data retrieval tools suggested by the database updating centers are relatively simple for use of cellular and molecular investigators, but many prediction tools are based on understanding algorithms. This is the evidence, that the data management is related to mathematics and computer sciences in bioinformatics. There is a gap on the interest of biologists and biochemists to the use of prediction tools, since a little information is observed on designing algorithms. Computational biology is a scope more considered by investigators; however, the mathematics schemes are also used in their flowcharts.
|It is obvious that the progressions in bioinformatics are ratelimited; it depends on the application of new matrices in algorithms and elevation of mathematical instruments. The basic question is that, “what should molecular and cellular investigators know about algorithms and their updated versions?”
|On the excellent idea to know the details of bioinformatics algorithms, biologists and biochemists should spend the long and notgracious educational terms on mathematical aspects of computer and information sciences. In addition, they should refresh your information on update of tools.
|I thought these are the most important causes of investigators’ unwilling to apply bioinformatics tools in their studies. My opinion is that a biologist or a biochemist should know only common descriptions and updates about algorithms designed for tools, in different branches of bioinformatics including sequence homology, genomics, transcriptomics and structural modeling.
|Bioinformatics algorithms are step-by-step procedures for predicting an event. There are a set of mathematical and biological rules that support a prediction on calculating, processing and reasoning. The principles of each algorithm are commonly based on the two models; homology and ab initio. The models are updated on the development of mathematical schemes, whose details are not essential for a biologist to know. Although, the precision of updates can be considered on checking the outputs with quality control factors, but the link of tools within layers of a neural network point out that there are needs to know the descriptive characteristics of updated versions. The most important changes are primarily considered in the scoring matrices of structural and functional units (amino acid, base, rotamer), and then, the programs that try to relate each unit to its flanking sections.
|The primary scoring matrices are obtained on the molecular distance/similarity and conservation. Other soft matrices such as Position-Specific Scoring Matrix (PSSM) are also created and corrected on the primary data and score weights .
|DNA matrices were not complicated on the base substitutions;however, there were constitutional tendencies on the transition and transversion changes. PAM (Point Accepted Mutation) amino acid matrix , developed by Margaret Dayhoff was based on the evolutionary model, while BLOSUM matrix (BLOcks of Amino Acid SUbstitution Matrix), developed by Henikoff and Henikoff  was created on the BLOCK identity.
|The scores to predict the secondary structures were created by Chou- Fasman, namely the Chou-Fasman method , and were developed by Gor method . Furthermore, some scores were suggested on the backbone-dependent rotamers , and fold  recognitions for protein 3D prediction.
|Development of Programs
|The next generations of algorithms were developed on the Dynamic Programming and Hidden Markov Models (HMM). Furthermore, they were assimilated with other predicted documents on the homology methods [8,9].
|In conclusion, I thought the biological hypotheses can be supported using bioinformatics tools, without the consideration of mathematical schemes, so that the prediction results may convert the two-way hypotheses into the one-way ones, and confirm the results obtained of experimental studies.
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