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Comparison between Immunological and Molecular Based Methods for Diagnosis of Mycobacterium Infections in Cattle, Buffaloes and Human in Egypt
Elsayed MSA1*, Elkerdasy AF2,3, Akeila MA4 and Elsayed AA51Department of Bacteriology, Mycology and Immunology, University of Sadat City, Minufyia, Egypt
2Department of Biochemistry, University of Sadat City, Minufia, Egypt
3Department of Biomedical Science, College of Pharmacy, Shaqra University, Al-Dawadmi, Saudi Arabia
4Department of Microbiology, Alexandria University, Egypt
5Department of Internal medicine and Animal Infectious Diseases, Cairo University, Egypt
- *Corresponding Author:
- Elsayed MSA
Department of Bacteriology
Mycology and Immunology
University of Sadat City, Egypt
Tel: +20 48 2607037
E-mail: mohamed.sabry@vet.usc.edu.eg
Received date: August 03, 2016; Accepted date: August 22, 2016; Published date: August 27, 2016
Citation: Elsayed MSA, Elkerdasy AF, Akeila MA, Elsayed AA (2016) Comparison between Immunological and Molecular Based Methods for Diagnosis of Mycobacterium Infections in Cattle, Buffaloes and Human in Egypt. Cell Mol Biol 62: 125. doi: 10.4172/1165-158X.1000125
Copyright: © 2016 Elsayed MAS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Pathogenic mycobacteria are notorious for infections in animals and human their diagnosis hampered by atypical types. Animal products consumption is responsible for majority of diseased cases worldwide. Comparison between diagnostic tests appears to be lacking in Egypt. Therefore, this study aimed to evaluate diagnostic values of Flow-cytometry, Immuno-chromatography, Low-cost Density Microarray (LCD) array, High-Performance Liquid Chromatography (HPLC) and multiplex Polymerase Chain Reaction (PCR). Comparative Intradermal Tuberculin Test result was 1.31%. M. bovis and M. kansasii were high from animal samples while, M. chelonae and M. malmoense from human samples using LCD array, HPLC and multiplex PCR confirmation. Serological tests estimated with differences regarded to different antigens. Chembio DPP VetTB Assay gave the highest sensitivity result 94.8% while, TB-ST (Tuberkulose Schnell test) test kit showed the lowest 82%. Flow-cytometry, CD2, CD4, CD8 and δγ WC1+ cells were high in tuberculin-positive cases and low in negative proving pathogenic mycobacterial infections of tuberculin-positive cases. (LCD) array, (HPLC) and multiplex (PCR) proved sensitive discriminatory methods while; serologic assays and flow-cytometry represent rapid diagnostic tools. Further investigations required to improve the sensitivity and specificity of these tests. These results elucidate the importance of different diagnostic tests and considered backbone for future researches.
