alexa In Vitro Antimicrobial Activity of Crude Leaf Extracts

Journal of Traditional Medicine & Clinical Naturopathy
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Research Article

In Vitro Antimicrobial Activity of Crude Leaf Extracts from Aloe secundiflora, Bulbine frutescens, Vernonia lasiopus and Tagetes minuta against Salmonella typhi

Rachuonyo HO1*, Ogola PE2, Arika WM2, Nyamai DW2 and Wambani JR3

1Department of Microbiology, Kenyatta University, Kenya

2Department of Biochemistry and Biotechnology, Kenyatta University, Kenya

3Department of Medical Laboratory Sciences, Kenyatta University, Kenya

Corresponding Author:
Rachuonyo HO
Department of Microbiology
Kenyatta University, Kenya
Tel: 254715407214
E-mail: [email protected]

Received date: January 27, 2016; Accepted date: February 09, 2016; Published date: February 15, 2016

Citation: Rachuonyo HO, Ogola PE, Arika WM, Nyamai DW, Wambani JR (2016) In Vitro Antimicrobial Activity of Crude Leaf Extracts from Aloe secundiflora, Bulbine frutescens, Vernonia lasiopus and Tagetes minuta against Salmonella typhi. J Tradi Med Clin Natur 5:187. doi:10.4172/jtmcn.1000187

Copyright: © 2016 Rachuonyo HO, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Four medicinal plants leaves were investigated to evaluate their antibacterial potential of the methanol extracts against Salmonella typhi by disc diffusion method. The methanol extract from Aloe secundiflora showed strong antibacterial activity against a clinical isolate of Salmonella typhi at low concentrations (5.5 mg/ml) as compared to Bulbine frutescens extract (8.8 mg/ml). The minimum inhibitory concentration ranged from 5 mg/ml - 9 mg/ml whereas the maximum bactericidal concentration range from 7 mg/ml - 11 mg/ml. The standard antibiotic used was ciprofloxacin (15 μg/ml) was used as a positive control while dimethyl sulphoxide and distilled water were used as the negative control. The extracts were preliminary screened for the presence of secondary metabolites to determine the presence of flavonoids, alkaloids, tannins and saponins. The results supported the use of the medicinal plants in the treatment of infections caused by Salmonella typhi.


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