Measurement of Pancreatic Polypeptide and Its Peptide Variant in Human Serum and Plasma by Immunocapture-Liquid-Chromatography-Tandem Mass Spectrometry. Reference Intervals and Practical Assay Considerations.*Corresponding Author: Alan L Rockwood PhD, ARUP Laboratories, 500 Chipeta Way Salt Lake City, Utah 84132, USA, Tel: 801-213-2934, Fax: 801.584.5109, Email: [email protected]
Received Date: Jul 25, 2014 / Accepted Date: Aug 14, 2014 / Published Date: Aug 21, 2014
Citation: Escobar H, Kushnir MM, Ray JA, Merrell MA, Gomez G, et al. (2014) Measurement of Pancreatic Polypeptide and its Peptide Variant in Human Serum and Plasma by Immunocapture-Liquid-Chromatography-Tandem Mass Spectrometry. Reference Intervals and Practical Assay Considerations. Biochem Physiol 3:140.DOI: 10.4172/2168-9652.1000140
Copyright: © 2014 . This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Pancreatic polypeptide (PP) is secreted by pancreatic islets after ingestion of food. Meal composition is associated with concentration of PP released in circulation. PP can be elevated in blood of patients with endocrine pancreatic tumors, supporting its use as a biomarker. We developed an Immunocapture-Liquid Chromatography- Tandem Mass Spectrometry (IC-LC-MS/MS) assay to quantify Pancreatic Polypeptide (PP) and its metabolite, PP3-36, in human serum or plasma. PP and PP3-36 were enriched from serum or plasma using immunopurification and the samples were analyzed using LC-MS/MS (AB SCIEX 5500) Total imprecision of the method was less than 20%; LOD and LOQ for PP and PP3-36 were 5 pg/mL and 10 pg/mL, respectively. Reference intervals in healthy subjects were <265 pg/mL (fasting); and 20 to 639 pg/mL (postprandial). Regression analysis for the comparison with a commercial RIA method showed poor agreement. Different types of meal increase the peptides levels differently; enhanced secretion of PP and PP3-36 was observed after meals rich in either protein and fat, or carbohydrates and fat, as compared with meals containing only carbohydrates. No relationship was observed between PP and body mass index or body fat. In summary, a direct LC-MS/MS method for determination of PP and PP3-36 in serum and plasma was developed and validated. The only endogenous truncated form of PP detected along with the intact PP in patient samples was, PP3-36, Our data suggest association of the meal type with secretion of PP; associations between concentrations of PP and body mass index or body fat percentage were not observed.