Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Production and Characterization of Streptokinase Enzyme by Using Streptococcus mutans Strain in Liquid State Fermentation through Corn Steep Liquor (CSL) Substrate

Abdul Ghaffar*#, Bilal Ahmed*#, Bushra Munir, Rana Faisal and Zahid Mahmood
Department of Applied Chemistry and Biochemistry, Main Allama Iqbal Road, Government College University, Faisalabad, Pakistan
#Authors contributed equally
Corresponding Authors : Abdul Ghaffar
Department of Applied Chemistry and Biochemistry
Government College University, Faisalabad, Pakistan
Tel: +92 41 9200037
E-mail: aghaffaruaf@yahoo.com
  Bilal Ahmed
Department of Applied Chemistry and Biochemistry Government College University
Faisalabad, Pakistan
E-mail: bilalahmed814@gmail.com
Received September 04, 2015; Accepted September 22, 2015; Published September 29, 2015
Citation: Ghaffar A, Ahmed B, Munir B, Faisal R, Mahmood Z (2015) Production and Characterization of Streptokinase Enzyme by Using Streptococcus mutans Strain in Liquid State Fermentation through Corn Steep Liquor (CSL) Substrate. Biochem Physiol 4:178. doi: 10.4172/2168-9652.1000178
Copyright: © 2015 Ghaffar A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Related article at Pubmed, Scholar Google

Abstract

Background: Enzymes play a very important and central role in the chemical reactions, occurring in biological systems. The study of enzymes has an immense practical importance. They have high catalytic power, many times greater than the synthetic catalysts. Vascular blockage can cause serious consequences leading to death by a thrombus (blood clot) developed in the circulatory system. Streptokinase is an extracellular protein Trans-located by many strains of beta-hemolytic Streptococci. By converting the plasminogen, to fibrin lytic enzyme, plasmin, it favor’s the blood clot lysis.

Results: The main objective of this research was the production of streptokinase in liquid state fermentation using CSL substances as substrate from Streptococcus species which was grown on blood agar media. Streptococcus mutans was selected for streptokinase production using corn steep liquor as substrate. Corn steep liquor (CSL) was applied in different concentrations ranging from 0.1% to 0.8% in liquid state fermentation culture medium and fibrin clot lysis method was used for enzyme assay.

Conclusion: Among different concentrations of CSL 0.3% was selected as optimum concentration, 44% blood lysis was observed by CSL in liquid state fermentation culture medium for streptokinase production.

Keywords

Google Scholar citation report
Citations :

Biochemistry & Physiology: Open Access received citations as per Google Scholar report

Indexed In
  • CAS Source Index (CASSI)
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • JournalTOCs
  • Ulrich's Periodicals Directory
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • EBSCO A-Z
  • OCLC- WorldCat
  • Scholarsteer
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Euro Pub
  • ICMJE
Share This Page
Top