A Comparative Study On Proteomic And Biochemical Alterations In The Cyanobacterium Anabaena Sp. PCC 7120 Under Short Term Exposure Of Abiotic Stresses: Pesticide, Salinity, Heavy Metal And UV- B | 4630
Journal of Biotechnology & Biomaterials
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Cyanobacteria are significant contributors to global photosynthetic productivity, thus making it relevant to study how the
different abiotic stresses can alter their cellular activities. Here, changes in phycocyanin pigment composition, antioxidants
enzyme activity, peroxidase production, NADPH, thiol content and SDS-PAGE protein profile were examined during a short-
term exposure of the cyanobacterium Anabaena sp. strain PCC7120 to Salinity, Butachlor, Cadmium and UV-B. Of these stress
conditions, a maximum inhibiton of phycocyanin content was observed for UV-B irradiation followed by butachlor, salt and Cd as
compared to control. The total thiol content in the organism is raise slightly over the basal level, to overcome stress imposed by salt
and cadmium. However, in UV-B and butachlor only showed the 0.6 and 0.95 fold significant decline in thiol level in comparison
to control. Interestingly, in all cases, NADPH\NADH level is significantly decrease below the basal level. Lipid Peroxidation
was found to be increase significantly in all stresses, while a SOD and catalase registered a maximum increase in cadmium and
UV-B. A peroxidase production found to be highest in response to butachlor. Further, SDS-PAGE analyses of the total protein
profile revealed the appearance of several differentially expressed protein bands in all the four stress. In addition, several up and
down regulated proteins, newer protein bands appeared in treated cultures when compared to the control. However, biochemical
and proteomic attributes suggests that, to overcome the abiotic stress, organisms generally might have defense mechanisms that
prevent interaction with the stress factors and that counteract the stress-induced damages.
Dhammika Leshan Wannigama is the final year student of Bsc (Hons) Industrial Microbiology at Center for Advanced studies in Botany, Banaras
Hindu University, Varanasi-221005, India. He has published three international research publications, two seminar publications and including final
year dissertation project related to molecular biology, under supervision of Professor L.C Rai. In year 2011 he has won the summer research training
fellow ship in Tropical Microbiology, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand.
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