A Forward Genetic Approach In Arabidopsis Thaliana Identifies Novel Crucial Genes And Mechanisms For Seed Longevity | 17049
Journal of Biotechnology & Biomaterials
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A forward genetic approach in Arabidopsis thaliana identifies novel crucial genes and mechanisms for seed longevity
5th World Congress on Biotechnology
Eduardo Bueso, Ramón Serrano, Jesús Muñoz-Bertomeu, Liliam Martínez, Enric Sayas, Patricia Ballester1, Lynne Yenush, Carla Ibañez, Gaetano Bissoli,
Miguel Gonzalez-Guzmán, Pedro L Rodriguez, Krzysztof Rakowski, Veronique Brunaud and Francisco Campos
Seed longevity is important to preserve crop and wild plants but it is limited by progressive cellular damage during storage.
Seeds are supposed to be protected against aging by cellular defenses and by structures such as the seed coat. However,
blind genetic screens to identify the crucial genes for seed longevity have never been made. We have screened an activation-
tagging mutant collection of Arabidopsis thaliana and selected four dominant mutants with improved seed longevity (isl1 to
4-1D) under both natural and accelerated aging conditions. In the isl1-1D mutant the longevity is caused by over-expression
of the transcription factor ARABIDOPSIS THALIANA HOMEOBOX 25 (ATHB25; At5g65410), that increases the expression
of gibberellic acid 3-oxidase 2 (GA3OX2), encoding a gibberellins (GA) biosynthetic enzyme, and the levels of GA1 and GA4
are higher (3.2- and 1.4-fold, respectively) in the mutant than in wild type. Seeds from wild type plants treated with GA and
from a quintuple DELLA mutant (with constitutive GA signaling) are more tolerant to aging, providing additional evidence
for a role of GA in seed longevity. In the isl2-1D mutant the longevity is caused by over-expression of the RING-finger of seed
longevity (RSL1; At2g26130) and morphological alterations suggest increased GA responses despite unaltered GA levels. In
both mutants reciprocal crosses demonstrated a maternal effect and, together with the altered morphology of the seed coat,
this suggests that our novel determinants of seed longevity reinforce the seed coat and probably reduce oxygen permeability.
Eduardo Bueso is Agricultural Engineer and Researcher at the Institute of Plant Molecular and Cellular Biology of the Polytechnic University of Valencia, Spain.
He works on the molecular mechanisms of seed longevity and has recently published two crucial articles on a novel approach to improve this crucial parameter in
agriculture and bioconservation.
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