Journal of Biotechnology & Biomaterials
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Plant materials are emerging as an exciting resource for the development of non-toxic noble nanoparticles. In this study, we
demonstrated a single step method for synthesis of silver chloride nanoparticles (AgCl-NPs) using leaf extract of Cissus
quadrangularis under ambient condition. It was found that aqueous AgNO3 when treated with leaf extract of C. quadrangularis
was reduced leading to the formation of AgCl-NPs which was continuously monitored by measuring the change in absorbance
at different time intervals using UV?visible spectral studies. The synthesized particles were characterized by FTIR, XRD and FESEM
to understand the nature, size and shape. The average size of the AgCl-NPs was found to be 13 nm. The antibacterial activity
of AgCl-NPs was evaluated against Gram positive and Gram negative bacterial pathogens by minimum inhibitory concentration
(MIC) test. Results showed that AgCl-NPs could inhibit the growth of tested bacteria and their MIC values were less than 7 μg/
mL. Thus, the leaf extract of C. quadrangularis could be used as a bio-reactant for the biogenic synthesis of AgCl-NPs and it can
be used as potent antibacterial agent.
P. Velusamy has completed his Ph.D from Centre for Advanced studies in Botany, University of Madras. At present he is serving as faculty member
in the Department of Biotechnology, SRM University, India. He has served as postdoctoral fellow in the Department of Biomedical Sciences at
Academia Sinica, Taiwan and Department of Biotechnology at Chonnam National University, South Korea during 2004-2009. He is actively involved
in, to explore the mechanism of antimicrobial activity with using biogenic nanoparticles. He has published 20 research papers, in reputed journals
and serving as an editorial board member of repute.
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