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A Preliminary In-vitro Study Of The Carbon Mesh As A Scaffold For Growth Of Fibroblasts | 4656
Journal of Biotechnology & Biomaterials
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Tissue engineering is the process of creating functional 3-D tissue combining cells with scaffolds that facilitate cell growth,
organization and differentiation. The most important aspect of tissue engineering is the adhesion and proliferation of cells on
scaffold material. The cells are grown on the scaffold that functions to provide mechanical support for cells and synchronize the
functions of cells in a manner analogous to extracellular matrix. Carbon as an inert element has advantages over other materials
because it is a basic constituent of tissues. The high proportion of the tissues of living organisms is composed of carbon compounds
so it should be tolerated by the tissues. The fibroblasts are common cells present in the connective tissue that synthesizes and
continuously secretes precursors of extra cellular matrix. Fibroblasts provide a structural framework for many tissues and play
a critical role in wound healing. To develop a novel biomaterial for wound healing in-vitro biocompatibility of carbon mesh is
tested. In the present study, carbon mesh is cut in desired size and after sterilization placed in six well cell culture plates. The
mesh was co-cultured with mouse embryonic fibroblast (MEF) cells. At different time intervals the viability and proliferation of
the MEF cells was evaluated using Phase contrast inverted microscopy, Light microscopy and Scanning electron microscopy. The
results will be discussed in detail at the time of presentation.
Mamta Negi completed her Master in Biotechnology with specialization in Animal Biotechnology from Allahabad Agricultural Institute Deemed
University, Allahabad, Uttar Pradesh and is pursuing PhD from the same Institute. She has published papers in reputed journals and some are
under communication. Presently, she is working as JRF in the DBT sponsored project entitled ?Development of the 3-D biodegradable matrices for reconstructive surgery? investigated by Dr Naveen Kumar in the Division of Surgery, Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh.
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