Journal of Biotechnology & Biomaterials
Open Access

Abstract

We propose a label free, non-invasive and efficient immunosensing platform to investigate an oral cancer biomarker (CYFRA-21-1) that is secreted in saliva sample. For the fabrication of this immunosensor, Yttrium Oxide (Y2O3) was used as an interfacing material synthesized through hydrothermal process. Further, 3-aminopropyl triethoxy silane (APTES) was used to functionalize Y2O3 nanoparticles. Electrophoretic deposition technique was used to deposit functionalized Y2O3 (i.e., APTES/Y2O3) on indium tin oxide coated glass (ITO) electrode at 50 V for 60 sec. For efficient covalent immobilization of anti-CYFRA-21-1 on APTES/Y2O3/ITO, EDC/NHS chemistry was utilized. Synthesized nanostructured Y2O3 and immunoelectrode was characterized by X-ray diffraction (XRD), atomic force microscopy (AFM), fourier transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM) and cyclic voltammetry (CV) techniques. The electrochemical response studies reveal that the immunosensor can be used to estimate CYFRA-21-1 biomarker in a wide linear detection range (2 to 22 ng mL-1), has high sensitivity (9.8×10-3 mA mL ng-1 cm-2) and remarkable lower detection limit (0.218 ng mL- 1) with shelf life of up to 7 weeks. The results of these studies have been validated via enzyme linked immunosorbent assay (ELISA). The results of these studies reveal the efficacy of the BSA/anti-CYFRA-21-1/APTES/Y2O3/ITO platform as a high throughput compact biosensing device for investigating CYFRA-21-1 biomolecules.

Biography

Email: shwetapanwar086@yahoo.com