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A Study On Protease Enzyme From Halophylic Microorganisms | 4876
Journal of Biotechnology & Biomaterials
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Studies carried out on halophilic and non-halophilic microorganisms, especially during the last decade, have increased
our current knowledge about different aspects, such as their physiology, ecology, taxonomy or phylogenetic relationship
with other microorganisms and, to a lesser extent to their genetics. Besides ,there are several fields in which there industrial
application are more promising as in the case of other extremophilic microorganisms and alkalophilic microorganisms they have
an important biotechnological potential as a source of compatible, solute enzyme and other compound of industrial interest.
More over moderately halophilic microorganism constitute an excellent model for the study of osmoregulatory mechanism that
permits them to grow wide ranges of salt concentration. This aspect has very exciting potentialities such as, for instance, they can
be used as alternative for the chemical treatment, most of these extremophiles are able to produce high concentration of enzyme
at extreme condition, which can be used for the degradation of xenobiotics, their possible application in medical industry for
the removal of blood stain and various surgical instruments. Also potential application in silver stripping from used film and as
biocleansing agent in detergent industry.
In the present study an effort is done to isolate and characterise the microorganisms from the halophilic soil sample. The
enzyme produced by the organisms Bacillus subtilis and Aspergillus flavus were subjected for the enzymatic studies. So it
indicated that the enzyme is thermo stable halo tolerant and alkaline protease. The isolates had shown different enzyme activity
with substrate casein and hair. The enzyme showed compatibility with detergent as biocleansing agent and removal of blood stain
from surgical instrument indicated great importance in the detergent industry and medical uses for sterilization.
Annapurna S. Agasthya is working currently as Professor of Microbiology and Bioprocess engineering of Lovely Professional University and has
worked at various capacities and the research interests include the Medical Microbiology, Immunology, Enzymology, Medical Mycology, Parasitology,
Food and Environmental Microbiology. She has guided the students for B.Tech, M.Tech. and M.Phil Biotechnology and Post Graduate students for
their dissertation. Presented the papers in various National and International conferences as well as published papers in various reputed journals.
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