A Suggestion About Rapid Rational Conditions Of CNS Safety From Various Vaccine Strategies | 71852
Journal of Infectious Diseases & Therapy
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The most realistic way before the recent epidemic that occurred in unexpected times and places in the world is rapid
supplying of vaccines related with the infectious diseases within limited times. However, little considerations about rapid
supplying a precise manual for the safety assurance of central nervous system (CNS) were established well so far. Thus, this
careless in the development of general and/or emergent vaccines should be corrected with a certain secure of safety protocol
which can reduce the risks on CNS damages before distribution. Hereby, the present study is undertaken to establish a manual
which a certain material can make CNS damage such as breaking down blood-brain barrier (BBB) protecting brain. Since
BBB is a critical morphological structure which is selectively permeable between blood vessels and brain, it would be very
important to know which conditions (i.e. post-injection, -time) can make BBB vulnerable by pyrogenic inflammatory agent
such as lipopolysaccharide (LPS) systemic injection. Following IP administration of the LPS to the mice, the mRNA levels of
typical markers of the damaged BBB tight junction such as ZO-1 and CLDN 5 were checked out. Based on conditions in the
LPS, IV Evans Blue should be administered after IP LPS administration according to each concentration (four conditions of
concentration) of LPS. BBB damages were able to be measured by Evans Blue existence checks by fluorescence wavelength
ranges from (ex: 620 nm/em: 680 nm) in the brain tissue. Ultimately, we could observe the mutual relations by comparison
with the two methods (mRNA and wavelength levels). According to the results, set LPS concentration can open BBB and by
the mRNA levels of tight junction, we can apply these results to general/emergency vaccine strategy.
Sun Shin Yi has completed his PhD from Seoul National University, Republic of Korea, and Post-doctoral studies from Marquette University, USA. Now, he is a Professor in the Department of Biomedical Laboratory Science, an Associate Dean of Special Affairs for Planning and Chair of IACUC at the Soonchunhyang University. He has published more than 60 papers in reputed journals, and is a Board Member of Korea Mouse Phenotype Center.
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