Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Google Scholar citation report
Citations : 2154

Journal of Biotechnology & Biomaterials received 2154 citations as per Google Scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • EBSCO A-Z
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
  • ICMJE
Recommended Journals
Share This Page

Advanced drug resistance SNP analysis and monitoring of liver physiological changes during Hepatitis B virus infection

3rd World Congress on Biotechnology

Samira Salehnejad, Mohammad Saeid Heidarnejad, Hamed Hemati Chahardeh and Thomas David Kumar

ScientificTracks Abstracts: J Biotechnol Biomater

DOI: 10.4172/2155-952X.S1.009

Abstract
Nucleoside analogue therapy allows the safe and long-term suppression of HBV. Lamivudine is a common drug for the treatment of chronic HBV patients. Resistant hepatitis B virus occurs as natural genome variables in YMDD motif of Lamivudine-treated and untreated chronic hepatitis B patients. Resistance detection is mainly accomplished by conventional lab methods and commercial kits. In this study we aimed to establish a Next Generation Sequencing?based method to detect Lamivudine-resistance by designing probes for a specific region of HBV genome. Meanwhile we monitored the physiological changes of liver before and during the treatment. Genomic DNA from serum samples of 92 patients with chronic hepatitis B virus infection was extracted. All patients were tested for liver enzymes, HbsAg, HbcAg, HbeAg, anti-HBs, anti-Hbe, anti- HBc, Bilirubin, Albumin and liver biopsy. NGS was accomplished using MINI-20 Sequencer by use of a mixture of reversible terminated dNTPs and Biotinylated DNA sense and antisense probes. In mix population status some mutants are not detected using conventional methods such as RFLP, nested-PCR or even common sequencing. Thus in resistant patient Lamivudine treatment is led to disease recurrence. Based on our findings Next Generation Sequencing could be more efficient, less time consuming and more sensitive for detection of mixed population of all YMDD motif mutants even rare single nucleotide mutation. It is also more accurate, faster, and more low-cost for patients.
Biography
Samira Salehnejad has completed her M.Sc. in Animal Physiology from Shahrekord University, IRAN. She co-founded PooyaGene Biotech Company, 2008 in IRAN as a research-driven and knowledge-based company, focusing on molecular diagnostic, stem cell and biopharmaceuticals research and manufacturing, with a branch in INDIA. She is already working as Senior Manager of PooyaGene Biotech Co.
Top