Journal of Biotechnology & Biomaterials
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Podophyllum hexandrum Royle belongs to the family Berberidaceae is an important Indian medicinal plant, which grows at
2700?4200 M in the Himalayan region. The rhizomes of this plant species are the source of podophyllotoxin, which is used
for the synthesis of anticancer drugs and for several medicinal applications. The Indian Podophyllum hexandrum is superior to
its American counterpart, namely, Podophyllum peltatum in terms of higher podophyllotoxin content (4% in the dried roots in
comparison to only 0.25% for Podophyllum peltatum). The seed looses viability and posses problem in regeneration in natural
habitat. In addition, the plants become endangered due to intensive collection and also owe its own biological characteristics.
Zygotic embryos were used as explant for in vitro germination. MS medium supplemented with GA
(5.0 mg l
) responded for in
vitro germination of embryos into plantlets. Establishment of adventitious root cultures was achieved using root explants derived
from in vitro seedlings. MS medium supplemented with IAA (3.0 mg l
) in combination with NAA (3.0 mg l
) was found to
be the optimal concentration for the induction of callus from the root explants. Maximum number of adventitious roots (14.1)
was obtained on MS Solid medium supplemented with IBA (1.5 mg l
). The Morphological differences of the root induced in
MS medium supplemented with IAA, IBA, IAA in combination with NAA has been observed and recorded. The fresh weight
and length of the roots increased in the IBA treatment when compared to the other hormones tested. The plant cell and tissue
culture technique discussed in this study is an alternative and a powerful protocol for the production of secondary metabolites.
Considering these fact, adventitious root culture in large scale regarded as an attractive alternative for production of secondary
metabolites of pharmaceutical and nutraceutical interest.
M. Rajesh is pursuing Doctoral degree in Biotechnology under the guidance of Dr. A. Ganapathi, Professor, Department of Biotechnology and
Genetic engineering, School of Biotechnology, Bharathidasan University, Tamil Nadu. He has experience in plant tissue culture and Agrobacterium
mediated genetic transformation in medicinal plants. At present he is concentrating in adventitious root culture for secondary metabolite production.
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