Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
GET THE APP
Agrobacterium Tumefaciens-mediated Genetic Transformation Of Black Night Shade (Solanum Nigrum L.) Using Root Explants | 4911
Journal of Biotechnology & Biomaterials
Like us on:
Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
An efficient variety-independent method for producing transgenic Black night shade (Solanum nigrum L.) via Agrobacterium
tumefaciens-mediated genetic transformation was developed. Root explants were transformed by co-cultivation with
Agrobacterium tumefaciens strain LBA4404 harbouring a binary vector pBAL2 carrying the reporter gene β-glucuronidase intron
(GUS-INT) and the marker gene neomycin phosphotransferase (NPTII). Transgenic calli were induced in media containing 0.1
mg l?1 thidiazuron (TDZ), 3.0 mg l?1 N6-benzylaminopurine, 100 mg l?1 kanamycin and 500 mg l?1 cefotaxime. The putative
transgenic shoot buds elongated on basal selection medium and rooted efficiently on Soilrite irrigated with water containing
100 mg l?1 kanamycin sulphate. Transgenic plants were raised in pots and seeds subsequently collected from mature fruits.
Histochemical GUS assay and polymerase chain reaction analysis of field-established transgenic plants and their off springs
confirmed the presence of the GUS and NPTII genes, respectively. Integration of T-DNA into the genome of putative transgenics
was further confirmed by Southern blot analysis. Progeny analysis of these plants showed a pattern of classical Mendelian
inheritance for both the NPTII and GUS genes.
T. Ugandhar has completed his Ph.D at the age of 25 years from Kakatiya University under the guideness of Prof N. Ramaswamy Head Department
of Biotechnology Kakatiya University Warangal immediately he was started his committed teaching career as a Lecturer in Botany at C.V. Raman
P.G. College Mancherial in 2005 and he was appointed as Assistant Professor by APPSC at S.R.R Govt Degree and P.G. College Karimnagar. He
has published 25 research articles, co-authored 2 books and guided 10 M.Phil theses. He has attended 15 national seminars and 3 international
seminars and also attends 4 symposia and workshops. His major fields of teaching and research include Cytology and Cytogenetics, Genetics
and Plant Breeding, Molecular Genetics, Mutation Breeding, Plant Tissue Culture and Biotechnology. He successfully applied mutation breeding to
brinjals, chillies and tomatoes and developed several agronomically useful varieties. He established clonal multiplication of certain forest trees. He
has a patent pending for In Vitro Propagation of Tassar Silk Plant.
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals