Journal of Biotechnology & Biomaterials
Open Access

Abstract

Industrial effluent is a major environmental threat due to contaminant loads, especially of heavy metals. Removal of these heavy metals from the contaminated sites has been a never ending challenge as they are persistent in the environment and cause major health issues. In recent days, a biotechnological approach i.e., biosorption by microbes has gained importance over the conventional methods. Fungal organisms and agro waste materials have received increasing attention in recent days as a potential biosorbent. The cell wall components (cellulose, hemicelluloses, chitin or pectin) along with functional groups like hydroxyl, carboxyl and amino groups of these biosorbents serve as active metal binding sites. Six species of Aspergillus, Penicillium sp. and a Trichoderma sp., exhibited resistance to Barium up to 2000 mM by well diffusion method. A species of Penicillium and two species of Aspergillus showed resistance to Lead up to 2000 mM. An adsorbent was designed by growing Aspergillus or Penicillium sp., on a natural agro waste substance i.e., loofah sponge under shaken condition for 48 hours at 37º C in broth supplemented with nutrients. The adsorption of Barium and Lead on dried adsorbent (loofah disc with fungal mycelium) showed 95% and 99% respectively as shown by Atomic Adsorption Spectrophotometer (AAS) analysis. Adsorption capacity of the designed adsorbent was further confirmed by Fourier Transform Infrared Spectroscopy (FTIR) and X-rays Fluorescence (XRF) analysis.

Biography

Sriharsha D V has completed his Masters in Microbiology from the Department of Microbiology and Biotechnology, Bangalore University in the year 2013. He is currently pursuing his PhD in the field of Environmental Microbiology from the same

Email: department. drsvtj@yahoo.co.in