'An Insight Into The Secondary Metabolite Profile Of Bergenia Ligulata Rhizome Showed Bergenin As The Most Potent Antilithiatic Agent | 40636
Journal of Biotechnology & Biomaterials
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The current lack in regimen of management of renal tissue calcification and the side-effects of synthesized allopathic
medicine shifted the focus of scientists towards the traditional system of herbal therapy. Bergenia ligulata is referred by
the ayurvedic system for treatment of kidney stone since decades but a scientific study indicating its metabolite responsible for
antilithiatic activity is lacking. The aim of the study was to isolate the most potent antilithiatic metabolite from the rhizome of
B. ligulata. In order to identify this, the crude extract of rhizome was fractionated using column chromatography guided by
in vitro calcium oxalate (CaOx) crystal growth inhibitory activity. The active fraction was characterized via LC-MS, FTIR and
NMR. Further, the in vitro antioxidant potential of purified molecule was also assessed. In vivo activity of the metabolite was
evaluated in hyperoxaluric rats given 0.4% ethylene glycol (EG) and 1.0% ammonium chloride (NH4Cl) for 9 days. Activity
guided fractionation led to the isolation of most potent antilithiatic metabolite from the rhizome of B. ligulata and spectroscopic
analysis revealed it as bergenin. At a dose of 10 mg/kg body weight of the treated rat, it protected against deleterious effects of
lithogenic treatment. Bergenin maintained oxidant/antioxidant balance in hyperoxaluric rats, thus mechanistic insight of its
antilithiatic activity was attributed to its antioxidant capability. The results of the present study provide significant evidence
that bergenin is an active component present in the rhizome of B. ligulata for managing CaOx calculi ameliorating renal injury.
Deepika Aggarwal is currently pursuing her PhD at the Department of Biochemistry, Panjab University. The aim of her research work is to investigate the antilithiatic potential of B. ligulata rhizome with a plunge to isolate the active metabolites responsible for this activity. She has got two publications with one original research article in Journal of Ethnopharmacology and another being a review to her credit. She has presented her work at many national and international conferences.