Journal of Biotechnology & Biomaterials
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In this study, survey and collected rhizospheric soil samples was done from different regions of Indogangetic plains in U.P.
(Gorakhpur, Lucknow, Kanpur, Varanasi, Meerut and Mau). Physiochemical properties of rhizospheric soil were determined
for their pH (ranges 7.0-8.4), EC-value (ranges 1.3-1.9ds/m) and organic carbon in soil (ranges 0.500-0.750 to 0.750-1.00% by
permagnate method). Isolation of rhizobacterial populations were made by the different inoculation techniques (soil plate and
serial dilution) on various general and specific culture media (Nutient agar, Jenson agar, Pikovshaya agar, Burk,s medium, NFB
medium, Malate Medium and YEMA medium). A total of 143 rhizobacterial isolates of from rhizospheric soil samples that
were visually characterized for their different morphotypes. As the different morphotypes were tested for the HCN production,
Siderophore production and Phosphate solubilization, in which some of the isolates showed significant results. Prominent plant
growth promoting rhizobacteria showing qualitative characteristics, further their samples were prepared in methanol and ethyl
acetate for the phenolic compounds analysis (Sinapic acid, Ferulic acid, Veratric acid, p-coumaric acid, Vanillic acid, Syringic
acid, p-hydroxybenzoic acid, Cinnamic acid, Benzoic acid, o-coumaric acid, m-coumaric acid, Caffeic acid, Salicylic acid,
Protocatechuic acid and Gentisic acid). Secondary metabolite profiling for the phenylpropanoids i.e phenolic compounds was
done to characterize rhizobacteria for the biomolecules production using HPLC standards. Some isolates were identified as
prominent phenolic compounds producers. Identification and quantification of these phenolic compounds in culture filtrates,
cell pellets and or in soil by these bacteria through LC-MS conditions is underway. During plant microbe?s interaction using
phenolic compounds i.e. biomolecules as plant growth promotion by the defence system activation, plant pathogens suppression
by production of inhibitory compounds and or symbiosis by the production of quorum sensing compounds in the ecosystem.
Therefore, identification and quantification of phenolic compounds producing rhizobacteria may helpful to develop consortia for
some metabolic interaction in rhizosphere that may influence plant growth and productivity.
Lalan Sharma has completed his Ph.D. from G.B. Pant University of Agriculture and Technology, Pantnagar in 2010 and worked on ?Variability in
Sarocladium oryzae and Management of sheath rot rice?. Obtained Jawahar Lal Nehru Memorial Award for his Doctoral Studies and also qualified
All India Level Competitions like ICAR-JRF, ICAR-SRF, and ICAR-NET. Qualified ARS and Joined as Scientist (Plant Pathology) at NBAIM in 2010.
He has published 4 research papers, 7 popular articles, and 5 proceedings chapters. He has participated in National conferences and also guided 4
M.Sc students. Presently, he is focusing on developing strategies to manipulate root-associated bacteria by estimation of PGPR and their secondary
metabolites i.e., phenolic compounds to improve soil health and crop productivity with eco-friendly safe.
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