alexa Analysis Of Promoters Recognized By Alternative Sigma Factors Of RNA Polymerase Using In Vitro Transcription System In Corynebacterium
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

2nd World Congress on Biotechnology
29 Nov � 1 Dec 2011 Philadelphia Airport Marriott, USA

Jiri Holatko
Posters: J Microbial Biochem Technol
DOI: 10.4172/1948-5948.S1.06
Abstract
Diff erent sigma subunits of bacterial RNA polymerase (RNAP) recognize diff erent classes of promoters. It is believed that Corynebacterium glutamicum promoters driving transcription of housekeeping genes are recognized by RNAP with the primary sigma factor SigA. Th e primary-like factor SigB is mainly involved in transcription of genes during the transition phase between exponential and stationary growth phases. SigC, SigD, SigE, SigH and SigM of C. glutamicum are members of the Group 4 sigma factors called also ECF (extracytoplasmic function) sigma factors. We characterized promoters of various classes by mapping the respective transcription start points, measuring their activity under various growth conditions by transcriptional fusions with the reporter genes and deriving the consensus sequences of specifi c classes. To determine, which sigma factors are involved in recognition of particular promoters, we have developed the fi rst in vitro transcription system for C. glutamicum. Th e in vitro transcription system consists of the promoter-carrying DNA template and RNAP holoenzyme reconstituted from the purifi ed His-tagged core RNAP and a separately isolated sigma factor. Using the in vitro assay, RNAP+SigA recognized specifi cally the vegetative promoters Pper from the C. glutamicum plasmid pGA1 and Pveg from Bacillus subtilis, whereas no transcriptional activity of RNAP+SigA on the SigH-dependent PdnaK promoter was observed. On the other hand, RNAP+SigH recognized specifi cally PdnaK promoter but not the vegetative promoters. In vitro transcription system may be particularly used for characterization of multiple promoters of diff erent classes and promoters recognized by two or more sigma factors.
Biography

Jiři Holátko (born 1979) completed his Ph.D. from Charles University in Prague in 2009. He participates on an international project analyzing sigma factors of RNA polymerase in Corynebacterium glutamicum at postdoctoral position in the Institute of Microbiology of Czech Academy of the Sciences. He has published 6 papers in international journals and is an author of 1 European patent.

image PDF   |   image HTML
 

Relevant Topics

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version