alexa

GET THE APP

Analytical Next-generation Sequencing Approaches For CRISPR-Cas9-mediated Cell Engineering | 97831
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Recommended Conferences

2nd International Conference on Biotechnology and Healthcare

Auckland, New Zealand

6th International Conference on Vaccines and Immunology

Geneva, Switzerland

3rd European Congress on Virology

Geneva, Switzerland
Google scholar citation report
Citations : 1600

Journal of Biotechnology & Biomaterials received 1600 citations as per google scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • EBSCO A-Z
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Share This Page

Analytical next-generation sequencing approaches for CRISPR-Cas9-mediated cell engineering

Joint Event on 24th Biotechnology Congress: Research & Innovations & Annual Congress on CRISPR Cas9 Technology and Genetic Engineering

Haythem Latif

GENEWIZ, USA

ScientificTracks Abstracts: J Biotechnol Biomater

DOI: 10.4172/2155-952X-C4-097

Abstract
CRISPR-Cas9-mediated cell lines hold great promise to transform therapeutics in various disease areas. Next-generation sequencing (NGS) is a powerful analytical tool with applications designed throughout the CRISPR-Cas9 workflow from discovery, through screening, and to validation of CRISPR-Cas9 effects. Here we will review contemporary workflows for CRISPR-Cas9 knockout libraries and briefly highlight how NGS and advanced informatics facilitates drug target identification. We will also detail various routes for optimizing and screening CRISPR-Cas9 pools and isolates for on- and off-target activity. Moving beyond screening and lead identification, we will review novel unguided NGS approaches to demonstrate on-target specificity while confirming low/no off-target activity prior to clinical trials.
Biography

Haythem Latif is a life science professional with a passion for next-generation sequencing technologies and applications. His interests in NGS began during his time as a graduate student pursing a PhD in Bioengineering from the University of California, San Diego. There, he generated, analyzed, and integrated multi-omic datasets to deeply characterize the genome organization of various microbial organisms in search of novel biological insights. Upon graduation, he worked for ThermoFisher in the Ion Torrent applications team focusing on AmpliSeq panel development. He is currently managing NGS Business & Strategic Partnerships at GENEWIZ.

E-mail: [email protected]

 

Top