alexa Angiotensin Converting Enzyme Inhibitory Activity In The Mealworm Tenebrio Molitor (Coleoptera, Tenebrionidae) Protein Hydrolysates
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Share This Page

Additional Info

Loading Please wait..

Joint Event on 15th World Congress on Biotechnology And Biotech Industries Meet and 2nd International Conference on Enzymology and Molecular Biology
March 20-21, 2017 Rome, Italy

Annarita Cito
Research Centre for Agrobiology and Pedology - CREA, Italy
Posters & Accepted Abstracts: J Biotechnol Biomater
DOI: 10.4172/2155-952X.C1.071
Hypertension is well known as one of the major risk factors for cardiovascular disease. The angiotensin converting enzyme (ACE) plays a key role in blood pressure regulation process. Hypertension treatment by synthetic ACE inhibitors (e.g. captopril, lisinopril, enalapril) is effective but their use can cause serious side effects, such as hypotension, cough, reduced renal function and angioedema. Therefore, research was focused on natural ACE inhibitory peptides sources such as foodstuffs and recently, also insects, promoted by the Food and Agricultural Organization of the United Nations (FAO) as a more environmentally sustainable, nutritious and functional alternative food to conventional livestock for human consumption. The purpose of this study is to investigate the ACE inhibitory activity in protein hydrolysates derived from the larval and pupal stages of the edible insect Tenebrio molitor (Coleoptera: Tenebrionidae). Each insect protein extract was hydrolyzed by the gastrointestinal enzymes (pepsin, trypsin and chymotrypsin) to simulate digestive process and compared to the crude extract. ACE inhibitory activity was measured by an indirect assay method based on the quantity of hippuric acid released by ACE from hippuryl-L-histidyl-leucine and determined by reverse-phase high performance liquid chromatography. Captopril was used as positive control and ACE inhibition degree expressed as the concentration of protein extract that inhibits 50% of ACE activity (IC50), assuming that the activity of the blank is equal to 100%. The IC50 value of captopril was 2.6x10-6 mg/mL. A significantly lower IC50 was detected after gastrointestinal hydrolysis of the protein extracts obtained from larvae (0.720 vs. 0.097 mg/mL after gastrointestinal hydrolysis) and pupae (0.484 vs. 0.132 mg/mL after gastrointestinal hydrolysis). Based on experimental data, T. molitor larvae represent the most promising development stage for the purification and identification of bioactive ACE inhibitory peptides, confirming the potential benefits of this coleopteran for human health.

Annarita Cito has completed her PhD in Biochemistry and Enzymology from the University of Siena (Italy) in 2010. Her dissertation investigated the role of homocysteine and some oxidative stress markers in neurodegenerative disorders (Alzheimer disease) and in autoimmune digestive disorders (such as celiac disease). She has expertise in cardiovascular disease mechanism and prevention. Currently, she is conducting research, as a Post-doctoral Researcher at CREAResearch Centre for Agrobiology and Pedology in Florence (Italy), on the evaluation of the potential use of the edible insect species Tenebrio molitor and Galleria mellonella as human diet supplement of polyunsaturated fatty acids and ACE inhibitory bioactive peptides for cardiovascular disease prevention.


image PDF   |   image HTML

Relevant Topics

Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

1-702-714-7001Extn: 9037

Business & Management Journals


1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version