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Antibacterial Activities Of Different Extracts Of Holoptelea Integrifolia | 4617
Journal of Biotechnology & Biomaterials
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The various parts of Holoptelea integrifolia, with vernacular name nemali chettu (telugu) a roadside plant, are indicated by
Charaka Samhitha, Sushrutha and other traditional systems for the treatment of inflammation, acid gastritis, Antitumor,
Intestinal Worms, Vomiting, Wounds, Leprosy, Antiviral, Diabetes, Adaptogenic, Anthelminitic. According to literature survey,
the plant Holoptelea integrifolia Roxb. exhibits a wide range of biological activities which have been reported by many scholars.
These all activities are subjected to further studies to establish the effective solvent and plant part. Methanol, Ethanol, Chloroform
and Aqueous extracts of the leaf of Holoptelea Integrifolia were studied for antibacterial activity against four bacterial strains using
agar well diffusion method and minimum bactericidal concentration and minimum inhibitory concentrations were determined
for each strain. The antibacterial activities of different extracts of Holoptelea Integrifolea at various concentrations were evaluated
where zone of inhibition was compared with the standard drug i.e ampicillin.
All extracts except aqueous extracts had a significant broad-spectrum inhibitory activity. The methanol extract of leaf had
the potent antibacterial activity, with a minimum inhibitory concentration (MIC) of 50μg/ml and a minimum bactericidal
concentration (MBC) of 350μg/ml against selected bacteria. The Methanolic extract of leaf showed maximum inhibitory activity
compared to Ethanolic, Chloroform and Aqueous extracts. Methanol extract of leaf was found to be very effective against all
the test organisms used. Ethanol extract of leaf was effective only against Staphylococus Aureus; Chloroform extract of leaf was
effective against E.Coli, B.Subtilis; aqueous extract of leaf was effective against S.aureus and E.coli, when compared to standard
drug ampicillin. The minimum inhibitory concentration for Methanol extract was found to be 50, 300, 25 and 100 μg/ml against
S.aureus, B.Subtilis, E.coli and S.typhi; for Ethanol extract was 100μg/ml (S.typhi); for chloroform extract was 100μg/ml (E.coli)
and 25μg/ml (B.subtilis) and for aqueous extract was 50μg/ml (S.aureus) and 25μg/ml (E.coli) respectively suggesting the
antibacterial activity of Holoptelea Integrifolia.
Kavitha Alli is pursuing her Ph.D from JNTU Hyderabad under the guidance of Dr. Laxmi Narasu and she completed her masters in BioTechnology
from Sri Shakthi Kailash women?s college, Salem under Periyar University, Tamilnadu. Her areas of interests include use of traditional medicinal
plants in modern medicine.
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