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Mango (Mangifera indica) is a fruit belonging to the genus Mangifera and family Anacardiaceae. Mango peels and seed
kernels are the major by-products of mango juice industry, they are rich sources of natural bioactive compounds which
play an important role in prevention of diseases. This study emphasized specifically on the potential of the mango Mangifera
Indica seed kernel by discovering the prospective usage of mango seed kernels as a source of antibacterial compounds against
Gram-positive (Staphylococcus aureus)and Gram-negative (Pseudomonas aeruginosa)bacterial strains. The significant increase
in mango consumption in domestic activity leads to the accumulation of waste, especially its kernel. This study attempts to screen
two varieties of mango kernels: Bannapalli and Senthura extracted using one extraction solvent i.e. distilled water to examine
the potential of mango kernel as natural antibacterial against two bacterial strains: Staphylococcus aureus and Pseudomonas
aeruginosa. Spread plate technique was employed to determine the antibacterial activity. Optimization of process conditions for
extraction of antibacterial activity (having low number of colonies in plates) was conducted in Triplicates observation methods
based on the experimental design by manipulating growth rate using kinetics and graphs. It was found that Bannapalli had
the best antibacterial activity, utilizing distilled water as the extraction solvent. The maximum antibacterial activity at 37?C
for 24h shows minimum number of colonies in plates. This finding would probably become an alternative source of new and
natural antibacterial agents. A mango kernel extract has a bacteriostatic and antibacterial activity, and thus can be used in food
products or cosmetics as a bacteriostatic and antibacterial agent. Furthermore, agents for preventing and treating acne or agents
for preventing dental caries can be provided by adding said extract as an effective component.
Alok Prakash is pursuing his Bachelors in Biotechnology at the age of 20, from VIT University, Tamilnadu, India. Currently he has completed his
second year of four years course. He is doing his summer internship from Britannia in Quality control department at Bangalore.
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