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In Association with
Appraisal of molecular phylogeny of Coccinia species employing chloroplast markers
6th World Congress on Biotechnology
Mala Parab and Sunita S
D Y Patil University, India
Posters-Accepted Abstracts: J Biotechnol Biomater
Abstract
Acumen of green plant phylogeny using chloroplast DNA molecular fingerprints has enlightened the liaison between character evolution and diversification at various levels of plant taxa. Among an array of chloroplast conserved core barcode sequences proposed by CBOL, rbcL, ycf5 and trnS-trnG have been used for inferring plant phylogenies. Coccinia spp. being, dioecious, facultative apomictic, vegetal crops disseminated in Asian and African continent. It has multifarious ethno-medicinal assets and is agronomically imperative crop. The present study attempts to resolve inter-/intra-phylogenetic relations, evolutionary trend and speciation pattern betwixt Coccinia spp. using few chloroplastid loci. Inconsequential genetic/ evolutionary distances within Coccinia spp. allude to possibility of reversal monophyly and high consanguinity percentage amid Coccinia within both the CpDNA loci i.e., trnS- trnG and rbcL. The rbcL and trnS- trnG loci, thus demonstrated chloroplast homoplasy, reticulate speciation pattern, LBAs and LDDs. However the trnS- trnG loci could cluster all Coccinia species in to one clade. A similar plesion group amidst Coccinia was reported from our previous study using phenetic and MLSPAP based genetic differentiators. Thereupon we conclude that rbcL are symplesiomorphic traits amid Cucurbitaceae members, while trnS-trnG are synapomorphic traits that can be used for resolving higher phylotaxas among Cucurbits. The ycf5 marker locus was efficant in resolving phylogeny at both infraspecies and infrageneric levels. We also propose that Coccinia spp. descendants of neutral theory of evolution, were observed to get enrouted towards parallel evolution and exhibit paternal inheritance.Biography
Mala Parab is currently pursiung her PhD at the School of Biotechnology and Bioinformatics, D Y Patil University, India. She is also being associated with the school as Assistant Professor since 9 years. She has also worked as an Associate Faculty, BSc (Biomedical Sciences and Molecular Biology), University of Central Lancashire, United Kingdom for two years She has published more than 5 papers in reputed journals and over 20 sequences to NCBI during her PhD.
Email: malaparab@gmail.com
