Journal of Biotechnology & Biomaterials
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is a Gram-negative bacterium, able to use only sucrose, glucose and fructose as carbon and energy
sources; sugar catabolism proceeds through the Entner-Doudoroff pathway, giving ethanol and CO
as final products.
Ethanol production using
in place of Saccharomyces cerevisiae has been object of many study, while the aptitude
to produce CO
for baking application, has rarely been considered. As for
used in bakery, the gas
evolved can be used to leaven dough. In recent years the incidence of baker?s yeast intolerance (due to an immune response
towards mannans) is rising, thus
can represent an interesting candidate to new products addressed to people having
adverse responses to traditional bakery foods. Fermentation trials aimed to optimize cultural conditions and biomass yield
were performed from flasks (300 mL- 1L) to pilot scale fermenter (14 L). A positive up-scaling effect was evidenced: from
around 0.8 g dcw/L in flasks to 1.4 g dcw/L in pilot plant fermenter. The biomass samples obtained at 9 and 16 h of fermentation
in bioreactor were employed in a dough-model system to evaluate their leavening performance, compared to
Leavening trials proved that
is able to rise a dough and also that it has a gas production rate (mL/g min) similar or
, reaching a higher total amount of gas evolved (Y End, mL/g). Results state the possibility of replacing
S. cerevisiae with
to create new yeast-free baked goods.
Alida Musatti has completed her PhD in 2012 in?Experimental and Clinical Nutrition? at the age of 27 years at the University of Milan (Italy). The research was
partially performed at the IATA-CSIC, Valencia (Spain). In 2013 she has obtained a Post-doctoral fellowship, still ongoing, at the University of Milan. Her scientific
activity is focused on the area of applied microbial biotechnology, concerning the set-up of biotechnological conditions for the production of microbial biomass,
evaluation of the antimicrobial activity of molecules suitable for active packaging and the application of biological pre-treatment to increase biomethane production
potential of lignocellulose material.
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