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Association Of DAPK1 Gene Methylation With The Prognosis In Epithelial Ovarian Carcinoma (EOC) Patients | 4610
Journal of Biotechnology & Biomaterials
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Background: Novel strategies for early detection of EOC, the most common and second most lethal cancer in Indian women, are
urgently needed. Silencing tumor suppressor genes via DNA methylation has established hypermethylation as one of the most
frequent molecular alterations that may initiate and drive many types of human neoplasia including EOC .To determine the
alterations of tumor suppressor gene DAPK1 gene in EOC patients to explore the possibilities of identifying potential minimally
invasive markers in blood of the patients, which could help in the clinical practice as a diagnostic and prognostic marker.
Fifty EOC patients with primary epithelial ovarian cancer were selected for the study; Genomic DNA extracted from
fresh peripheral blood & serum followed by sodium bisulfate modification .The p16 methylation was detected using methylation-
specific PCR (MSP). The DAPK1 gene methylation status was correlated with age, stage, menopause, Ca125.5 and clinic
The frequencies of DAPK1 gene methylation in EOC patients were found to be 68%. The significant association was seen
with age at diagnosis, menopause and stage (P = 0.043). Patients with high methylation indices had poor prognosis (p<0.001,
Hazards ratio=14.58) with age (P = 0.043), and tumor stage (P = 0.033). Aberrant methylation of DAPK1 gene was strongly
associated with EOC patients (P = 0.037).
Our results that the methylated loci of TSGs (DAPK1 gene) may be employed as clinically useful biomarkers for
prognosis and diagnosis of EOC noninvasively using readily available body fluid by MS-PCR and proved to be efficient and cost-
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