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At-line Monitoring Flow Cytometric Approach For Lipid And Carotenoid Detection In Yeasts | 4913
Journal of Biotechnology & Biomaterials
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Microbial oils can be used as feedstock for biodiesel production. Compared to other vegetable oils and animal fats, the
production of microbial oil has many advantages: short life cycle, less labor required and easier to scale-up. However,
at present, the major obstacle for commercialization of biodiesel obtained from microbial lipids is the high production cost
involved. Therefore it is crucial to explore approaches to reduce the price of microbial biodiesel process as the coproduction of
microbial lipids and high value added-products.
Biodiesel production from yeasts may have particular interest as these microorganisms may contain a high lipid content
which can be extracted and converted into biofuel.
In addition, some yeasts (Rhodotorula sp.) contain carotenoids of high commercial interest (beta-carotene, others) which
are used as natural food colorants and feed additives in aquaculture. The co-extraction of lipids and carotenoids from the yeast
biomass, in a biorefinary concept, will allow the economical sustainable biofuel production since the high-value added products
(carotenoids) will support the fuel production.
Therefore it is crucial to monitor the lipid and carotenoid production when producing these compounds from yeast. If at-line
information is available, it is possible to change the process control strategy during the process progress, in order to achieve the
maximum productivities by changing the operational conditions (agitation, aeration, medium composition, etc.). Such approach
is not possible when using conventional microbiology techniques such as optical density, dry cell weight or colony forming units,
currently used for process monitoring.
In the present work we used flow Cytometry to at-line monitor the lipid and carotenoid content in some Rhodotorula
species. Such approach allows the quick process optimization from bench to pilot scale.
Teresa Lopes da Silva is a Chemical Engineer, done her Ph.D. degree (Biochemical Engineering) from the University of ?vora in 2005. She is a
Researcher at the National Laboratory for Energy and Geology (LNEG). She is the head of the Flow Cytometry Group at the Bioenergy Unit/LNEG
and leads the Biotechnology Process Group of the Iberian Cytometry Society. She was awarded as a postdoc at the Birmingham University (UK) in
2006. She authored over 30 international peer-review scientific publications.
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