Biocatalytic Asymmetric Phosphorylation Catalyzed By Recombinant Glycerate-2-kinase | 60087
ISSN: 2155-952X

Journal of Biotechnology & Biomaterials
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
Recommended Conferences

6th International Conference on Vaccines and Immunology

Geneva, Switzerland

3rd European Congress on Virology

Geneva, Switzerland
Google Scholar citation report
Citations : 1600

Journal of Biotechnology & Biomaterials received 1600 citations as per Google Scholar report

Indexed In
  • Index Copernicus
  • Google Scholar
  • Sherpa Romeo
  • Open J Gate
  • Genamics JournalSeek
  • Academic Keys
  • ResearchBible
  • China National Knowledge Infrastructure (CNKI)
  • Access to Global Online Research in Agriculture (AGORA)
  • Electronic Journals Library
  • RefSeek
  • Hamdard University
  • OCLC- WorldCat
  • SWB online catalog
  • Virtual Library of Biology (vifabio)
  • Publons
  • Geneva Foundation for Medical Education and Research
  • Euro Pub
Share This Page

Biocatalytic asymmetric phosphorylation catalyzed by recombinant glycerate-2-kinase

13th Biotechnology Congress

Birhanu Mekuaninte Kinfu, Norman Hardt, Jennifer Chow, Bernhard Schoenenberger, Wolfgang R Streit, Markus Obkircher and Roland Wohlgemuth

University of Hamburg, Germany Merck Group, Switzerland

Posters & Accepted Abstracts: J Biotechnol Biomater

DOI: 10.4172/2155-952X.C1.068

D-glycerate-2-phosphate is an important substrate and crucial metabolite of central carbon metabolism, glycolysis/gluconeogenesis and pentose phosphate pathway, glycine, serine and threonine metabolism, methane metabolism, biosynthesis of plant secondary metabolites, phenylpropanoids, terpenoids and steroids, alkaloids derived from shikimate pathway, antibiotics, amino acids. Therefore an efficient, robust and scalable route for the preparation of enantiopure D-glycerate-2-phosphate is needed. A straightforward one-step biocatalytic phosphorylation of glyceric acid catalyzed by a recombinant glycerate 2-kinase heterologously expressed as maltose binding protein fusion has been investigated using racemic and the enantiopure D- and L-glycerate as substrate. The reaction was coupled with the phosphoenolpyruvate/pyruvate-kinase-system for ATP-regeneration and monitored by 31P-NMR spectroscopy. This phosphorylation reaction using recombinant glycerate 2-kinase is highly enantio-selective and sustainable, as it yields enantiomerically pure D-glycerate-2-phosphate in less reaction steps and with higher purity than chemical routes.

Birhanu Mekuaninte Kinfu has completed his BSc at the University of Gondar in Applied Biology and his MSc in Biotechnology at Addis Ababa University. He has worked as University Lecturer for 2 years. Winning the prestigious DAAD Research Grant Award (German academic exchange service) under its ‘young academics and scientists’ program, he is currently pursuing PhD at Microbiology and Biotechnology Department, the University of Hamburg, Germany. His main work focuses on biocatalytic phosphorylation of selected metabolites, metagenomics and in vitro protein expression systems.

Email: [email protected]